Literature DB >> 18670063

Verification of reference genes for relative quantification of gene expression by real-time reverse transcription PCR in the pig.

Katerina Svobodová1, Karel Bílek, Ales Knoll.   

Abstract

The aim of this study was to develop a set of reliable reference genes for quantification of mRNA expression in the pig. The mRNA expression stability in pig tissues was studied for 4 genes: EEF1A1, GAPDH, HPRT1 and TOP2B. The level of expression was characterized by Ct values for each gene and each tissue. By using the geNorm algorithm, the stability of the reference genes was determined in the diaphragm, heart, kidney, liver, lungs, longissimus muscle, and spleen. On the basis of this information, suitable reference genes can be selected for mRNA expression studies in relevant pig tissues.

Entities:  

Mesh：

Substances：

Year:  2008        PMID： 18670063     DOI： 10.1007/BF03195623

Source DB:  PubMed          Journal:  J Appl Genet        ISSN： 1234-1983            Impact factor:   3.240


  7 in total

1.  Differentiating embryonic stem cells: GAPDH, but neither HPRT nor beta-tubulin is suitable as an internal standard for measuring RNA levels.

Authors:  Christopher L Murphy; Julia M Polak
Journal:  Tissue Eng       Date:  2002-08

2.  Annotation of the Affymetrix porcine genome microarray.

Authors:  S Tsai; J P Cassady; B A Freking; D J Nonneman; G A Rohrer; J A Piedrahita
Journal:  Anim Genet       Date:  2006-08       Impact factor: 3.169

3.  Identification of novel universal housekeeping genes by statistical analysis of microarray data.

Authors:  Seram Lee; Minjoung Jo; Jungeun Lee; Sang Seok Koh; Soyoun Kim
Journal:  J Biochem Mol Biol       Date:  2007-03-31

4.  A compendium of gene expression in normal human tissues.

Authors:  L L Hsiao; F Dangond; T Yoshida; R Hong; R V Jensen; J Misra; W Dillon; K F Lee; K E Clark; P Haverty; Z Weng; G L Mutter; M P Frosch; M E MacDonald; E L Milford; C P Crum; R Bueno; R E Pratt; M Mahadevappa; J A Warrington; G Stephanopoulos; G Stephanopoulos; S R Gullans
Journal:  Physiol Genomics       Date:  2001-12-21       Impact factor: 3.107

5.  Development of a new set of reference genes for normalization of real-time RT-PCR data of porcine backfat and longissimus dorsi muscle, and evaluation with PPARGC1A.

Authors:  Tim Erkens; Mario Van Poucke; Jo Vandesompele; Karen Goossens; Alex Van Zeveren; Luc J Peelman
Journal:  BMC Biotechnol       Date:  2006-10-09       Impact factor: 2.563

6.  Selection of reference genes for gene expression studies in pig tissues using SYBR green qPCR.

Authors:  Ann-Britt Nygard; Claus B Jørgensen; Susanna Cirera; Merete Fredholm
Journal:  BMC Mol Biol       Date:  2007-08-15       Impact factor: 2.946

7.  Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes.

Authors:  Jo Vandesompele; Katleen De Preter; Filip Pattyn; Bruce Poppe; Nadine Van Roy; Anne De Paepe; Frank Speleman
Journal:  Genome Biol       Date:  2002-06-18       Impact factor: 13.583
  7 in total
  13 in total

1.  Porcine EEF1A1 and EEF1A2 genes: genomic structure, polymorphism, mapping and expression.

Authors:  Kateřina Svobodová; Pavel Horák; Antonín Stratil; Heinz Bartenschlager; Mario Van Poucke; Pavla Chalupová; Věra Dvořáková; Christoph Knorr; Roman Stupka; Jaroslav Čítek; Michal Šprysl; Anna Palánová; Luc J Peelman; Hermann Geldermann; Aleš Knoll
Journal:  Mol Biol Rep       Date:  2015-03-08       Impact factor: 2.316

2.  Evaluation of reference genes for studies of gene expression in the bovine liver, kidney, pituitary, and thyroid.

Authors:  Paweł Lisowski; Mariusz Pierzchała; Joanna Gościk; Chandra S Pareek; Lech Zwierzchowski
Journal:  J Appl Genet       Date:  2008       Impact factor: 3.240

3.  Transcriptional Characterization of Porcine Leptin and Leptin Receptor Genes.

Authors:  Dafne Pérez-Montarelo; Almudena Fernández; Carmen Barragán; Jose L Noguera; Josep M Folch; M Carmen Rodríguez; Cristina Ovilo; Luis Silió; Ana I Fernández
Journal:  PLoS One       Date:  2013-06-18       Impact factor: 3.240

4.  Identification of stable normalization genes for quantitative real-time PCR in porcine articular cartilage.

Authors:  Ryan S McCulloch; Melissa S Ashwell; Audrey T O'Nan; Peter L Mente
Journal:  J Anim Sci Biotechnol       Date:  2012-11-12

5.  Evaluation of potential reference genes for relative quantification by RT-qPCR in different porcine tissues derived from feeding studies.

Authors:  Qimeng Li; Konrad Johann Domig; Thomas Ettle; Wilhelm Windisch; Christiane Mair; Karl Schedle
Journal:  Int J Mol Sci       Date:  2011-03-07       Impact factor: 5.923

6.  Dynamic distribution and tissue tropism of classical swine fever virus in experimentally infected pigs.

Authors:  Jun Liu; Xue-Zheng Fan; Qin Wang; Lu Xu; Qi-Zu Zhao; Wei Huang; Yuan-Cheng Zhou; Bo Tang; Lei Chen; Xing-Qi Zou; Sha Sha; Yuan-Yuan Zhu
Journal:  Virol J       Date:  2011-05-02       Impact factor: 4.099

7.  Age-related changes in relative expression stability of commonly used housekeeping genes in selected porcine tissues.

Authors:  Muhammad Jasim Uddin; Mehmet Ulas Cinar; Dawit Tesfaye; Christian Looft; Ernst Tholen; Karl Schellander
Journal:  BMC Res Notes       Date:  2011-10-24

8.  Evaluation of suitable reference genes for gene expression studies in porcine alveolar macrophages in response to LPS and LTA.

Authors:  Mehmet Ulas Cinar; Mohammad Ariful Islam; Muhammad Jasim Uddin; Ernst Tholen; Dawit Tesfaye; Christian Looft; Karl Schellander
Journal:  BMC Res Notes       Date:  2012-02-18

9.  Selection of internal control genes for real-time quantitative PCR in ovary and uterus of sows across pregnancy.

Authors:  María Martínez-Giner; José Luis Noguera; Ingrid Balcells; Amanda Fernández-Rodríguez; Ramona N Pena
Journal:  PLoS One       Date:  2013-06-13       Impact factor: 3.240

Review 10.  Porcine endogenous retroviruses in xenotransplantation--molecular aspects.

Authors:  Magdalena C Kimsa; Barbara Strzalka-Mrozik; Malgorzata W Kimsa; Joanna Gola; Peter Nicholson; Krzysztof Lopata; Urszula Mazurek
Journal:  Viruses       Date:  2014-05-13       Impact factor: 5.048
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.