Literature DB >> 18664615

Towards a systematic validation of references in real-time rt-PCR.

Laurent Gutierrez, Mélanie Mauriat, Jérôme Pelloux, Catherine Bellini, Olivier Van Wuytswinkel.   

Abstract

Mesh:

Year:  2008        PMID: 18664615      PMCID: PMC2518241          DOI: 10.1105/tpc.108.059774

Source DB:  PubMed          Journal:  Plant Cell        ISSN: 1040-4651            Impact factor:   11.277


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  15 in total

1.  Relative expression software tool (REST) for group-wise comparison and statistical analysis of relative expression results in real-time PCR.

Authors:  Michael W Pfaffl; Graham W Horgan; Leo Dempfle
Journal:  Nucleic Acids Res       Date:  2002-05-01       Impact factor: 16.971

2.  Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper--Excel-based tool using pair-wise correlations.

Authors:  Michael W Pfaffl; Ales Tichopad; Christian Prgomet; Tanja P Neuvians
Journal:  Biotechnol Lett       Date:  2004-03       Impact factor: 2.461

Review 3.  Real-time PCR: what relevance to plant studies?

Authors:  Claire Gachon; Annaïck Mingam; Bénédicte Charrier
Journal:  J Exp Bot       Date:  2004-06-18       Impact factor: 6.992

4.  Real-time RT-PCR profiling of over 1400 Arabidopsis transcription factors: unprecedented sensitivity reveals novel root- and shoot-specific genes.

Authors:  Tomasz Czechowski; Rajendra P Bari; Mark Stitt; Wolf-Rüdiger Scheible; Michael K Udvardi
Journal:  Plant J       Date:  2004-04       Impact factor: 6.417

5.  Quantification of mRNA using real-time RT-PCR.

Authors:  Tania Nolan; Rebecca E Hands; Stephen A Bustin
Journal:  Nat Protoc       Date:  2006       Impact factor: 13.491

6.  The lack of a systematic validation of reference genes: a serious pitfall undervalued in reverse transcription-polymerase chain reaction (RT-PCR) analysis in plants.

Authors:  Laurent Gutierrez; Mélanie Mauriat; Stéphanie Guénin; Jérôme Pelloux; Jean-François Lefebvre; Romain Louvet; Christine Rusterucci; Thomas Moritz; François Guerineau; Catherine Bellini; Olivier Van Wuytswinkel
Journal:  Plant Biotechnol J       Date:  2008-04-22       Impact factor: 9.803

7.  Normalisation of real-time RT-PCR gene expression measurements in Arabidopsis thaliana exposed to increased metal concentrations.

Authors:  Tony Remans; Karen Smeets; Kelly Opdenakker; Dennis Mathijsen; Jaco Vangronsveld; Ann Cuypers
Journal:  Planta       Date:  2008-02-14       Impact factor: 4.116

8.  Heat-stress-dependency and developmental modulation of gene expression: the potential of house-keeping genes as internal standards in mRNA expression profiling using real-time RT-PCR.

Authors:  Roman A Volkov; Irina I Panchuk; Fritz Schöffl
Journal:  J Exp Bot       Date:  2003-10       Impact factor: 6.992

9.  The gibberellin biosynthetic genes AtGA20ox1 and AtGA20ox2 act, partially redundantly, to promote growth and development throughout the Arabidopsis life cycle.

Authors:  Ivo Rieu; Omar Ruiz-Rivero; Nieves Fernandez-Garcia; Jayne Griffiths; Stephen J Powers; Fan Gong; Terezie Linhartova; Sven Eriksson; Ove Nilsson; Stephen G Thomas; Andrew L Phillips; Peter Hedden
Journal:  Plant J       Date:  2007-12-06       Impact factor: 6.417

10.  Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes.

Authors:  Jo Vandesompele; Katleen De Preter; Filip Pattyn; Bruce Poppe; Nadine Van Roy; Anne De Paepe; Frank Speleman
Journal:  Genome Biol       Date:  2002-06-18       Impact factor: 13.583

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  94 in total

1.  Validation of reference genes for gene expression studies in peanut by quantitative real-time RT-PCR.

Authors:  Xiaoyuan Chi; Ruibo Hu; Qingli Yang; Xiaowen Zhang; Lijuan Pan; Na Chen; Mingna Chen; Zhen Yang; Tong Wang; Yanan He; Shanlin Yu
Journal:  Mol Genet Genomics       Date:  2011-12-28       Impact factor: 3.291

2.  Evaluation of candidate reference genes for expression studies in Pisum sativum under different experimental conditions.

Authors:  José V Die; Belén Román; Salvador Nadal; Clara I González-Verdejo
Journal:  Planta       Date:  2010-04-09       Impact factor: 4.116

3.  Comprehensive selection of reference genes for quantitative gene expression analysis during seed development in Brassica napus.

Authors:  Ronei Dorneles Machado; Ana Paula Christoff; Guilherme Loss-Morais; Márcia Margis-Pinheiro; Rogério Margis; Ana Paula Körbes
Journal:  Plant Cell Rep       Date:  2015-02-27       Impact factor: 4.570

4.  Evaluation and selection of reference genes for ecotoxicogenomic study of the green alga Closterium ehrenbergii using quantitative real-time PCR.

Authors:  Min-Ah Lee; Ruoyu Guo; Vinitha Ebenezer; Jang-Seu Ki
Journal:  Ecotoxicology       Date:  2015-02-28       Impact factor: 2.823

Review 5.  Functional analysis of transcription factors in Arabidopsis.

Authors:  Nobutaka Mitsuda; Masaru Ohme-Takagi
Journal:  Plant Cell Physiol       Date:  2009-05-28       Impact factor: 4.927

6.  Real-time quantitative RT-PCR: design, calculations, and statistics.

Authors:  Ivo Rieu; Stephen J Powers
Journal:  Plant Cell       Date:  2009-04-24       Impact factor: 11.277

7.  Assessment of reference genes for real-time quantitative PCR gene expression normalization during C2C12 and H9c2 skeletal muscle differentiation.

Authors:  Twinkle J Masilamani; Julie J Loiselle; Leslie C Sutherland
Journal:  Mol Biotechnol       Date:  2014-04       Impact factor: 2.695

Review 8.  Reference genes in real-time PCR.

Authors:  Bartłomiej Kozera; Marcin Rapacz
Journal:  J Appl Genet       Date:  2013-11       Impact factor: 3.240

9.  Selection of reliable reference genes for quantitative real-time polymerase chain reaction studies in maize grains.

Authors:  Vanessa Galli; Rafael da Silva Messias; Sérgio Delmar dos Anjos e Silva; Cesar Valmor Rombaldi
Journal:  Plant Cell Rep       Date:  2013-09-08       Impact factor: 4.570

10.  The interacting MYB75 and KNAT7 transcription factors modulate secondary cell wall deposition both in stems and seed coat in Arabidopsis.

Authors:  Apurva Bhargava; Abdul Ahad; Shucai Wang; Shawn D Mansfield; George W Haughn; Carl J Douglas; Brian E Ellis
Journal:  Planta       Date:  2013-01-18       Impact factor: 4.116

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