Literature DB >> 186634

Distinguishing cytomegalovirus, mycoplasma, and cellular DNA polymerases.

R L Miller, F Rapp.   

Abstract

Cytomegalovirus-induced DNA polymerase can be distinguished from infected-cell enzymes by activity in 100 mM (NH4)2SO4. Virus polymerase is stimulated to 145% of control, whereas mock-infected cell polymerase is inhibited to 12% of control without added salt. Mycoplasmas induce a DNA polymerase in cell extracts that is stimulated to 130 to 180% by 25 mM (NH4)2SO4. Mycoplasma DNA polymerase may be mistaken for a virus-induced polymerase when virus stocks are contaminated. Identification of virus, cellular, and mycoplasma DNA polymerases in total cell extracts is described using sedimentation rate and effect of inhibitors on DNA polymerase activities.

Entities:  

Mesh：

Substances：

Year:  1976        PMID： 186634      PMCID： PMC355032     

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  15 in total

1.  Nomenclature of eukaryotic DNA polymerases.

Authors:  A Weissbach; D Baltimore; F Bollum; R Gallo; D Korn
Journal:  Science       Date:  1975-10-24       Impact factor: 47.728

2.  DNA synthesis and DNA polymerase activity of herpes simplex virus type 1 temperature-sensitive mutants.

Authors:  G M Aron; D J Purifoy; P A Schaffer
Journal:  J Virol       Date:  1975-09       Impact factor: 5.103

3.  The effect of mycoplasmas on replication and plaquing ability of Herpes simplex virus.

Authors:  J E Manischewitz; B G Young; M F Barile
Journal:  Proc Soc Exp Biol Med       Date:  1975-03

4.  Human cytomegalovirus. III. Virus-induced DNA polymerase.

Authors:  E S Huang
Journal:  J Virol       Date:  1975-08       Impact factor: 5.103

5.  Characterization of herpes simplex virus-induced deoxyribonucleic acid polymerase.

Authors:  A Weissbach; S C Hong; J Aucker; R Muller
Journal:  J Biol Chem       Date:  1973-09-25       Impact factor: 5.157

6.  Envelope and nucleocapsid antigens of Cytomegalovirus (CMV).

Authors:  H Schmitz; H W Doerr; M Obrig
Journal:  Med Microbiol Immunol       Date:  1975-07-02       Impact factor: 3.402

7.  Synthesis of herpes simplex virus, vaccinia virus, and adenovirus DNA in isolated HeLa cell nuclei. I. Effect of viral-specific antisera and phosphonoacetic acid.

Authors:  A Bolden; J Aucker; A Weissbach
Journal:  J Virol       Date:  1975-12       Impact factor: 5.103

8.  DNA polymerase in pseudorabies virus infected cells.

Authors:  I W Halliburton; J C Andrew
Journal:  J Gen Virol       Date:  1976-01       Impact factor: 3.891

9.  Human cytomegalovirus. IV. Specific inhibition of virus-induced DNA polymerase activity and viral DNA replication by phosphonoacetic acid.

Authors:  E S Huang
Journal:  J Virol       Date:  1975-12       Impact factor: 5.103

10.  Differential effect of phenethyl alcohol on mycoplasmas and enveloped viruses.

Authors:  S P Staal; W P Rowe
Journal:  J Virol       Date:  1974-12       Impact factor: 5.103
View more
  4 in total

Review 1.  The mycoplasmas.

Authors:  S Razin
Journal:  Microbiol Rev       Date:  1978-06

2.  Isolation and characterization of phosphonoacetic acid-resistant mutants of human cytomegalovirus.

Authors:  R T D'Aquila; W C Summers
Journal:  J Virol       Date:  1987-04       Impact factor: 5.103

3.  Effects of the nucleoside analog 2'-nor-2'-deoxyguanosine on human cytomegalovirus replication.

Authors:  M J Tocci; T J Livelli; H C Perry; C S Crumpacker; A K Field
Journal:  Antimicrob Agents Chemother       Date:  1984-02       Impact factor: 5.191

4.  Acyclovir-resistant mutants of herpes simplex virus type 1 express altered DNA polymerase or reduced acyclovir phosphorylating activities.

Authors:  P A Furman; D M Coen; M H St Clair; P A Schaffer
Journal:  J Virol       Date:  1981-12       Impact factor: 5.103
  4 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.