BACKGROUND: The frequency of protective antiviral memory B cells after hepatitis B virus (HBV) vaccination is unknown. METHODS: A novel 2-step immunomagnetic protocol to assess the ex vivo frequency of protective HBV surface antigen (HBsAg)-specific memory B cells was used. RESULTS: HBsAg-specific memory B cells were detected in vaccinated individuals, although at very low frequency (median, 0.2% of CD19(+) cells [range, 0%-4% of CD19(+) cells]). No correlation existed between the frequency of HBsAg-specific memory B cells and the corresponding serum antibody titer or B cell enzyme-linked immunosorbent spot findings. CONCLUSION: Our results indicate sustained B cell-mediated protection against HBV despite waning antibody titers, which is consistent with clinical observations.
BACKGROUND: The frequency of protective antiviral memory B cells after hepatitis B virus (HBV) vaccination is unknown. METHODS: A novel 2-step immunomagnetic protocol to assess the ex vivo frequency of protective HBV surface antigen (HBsAg)-specific memory B cells was used. RESULTS: HBsAg-specific memory B cells were detected in vaccinated individuals, although at very low frequency (median, 0.2% of CD19(+) cells [range, 0%-4% of CD19(+) cells]). No correlation existed between the frequency of HBsAg-specific memory B cells and the corresponding serum antibody titer or B cell enzyme-linked immunosorbent spot findings. CONCLUSION: Our results indicate sustained B cell-mediated protection against HBV despite waning antibody titers, which is consistent with clinical observations.
Authors: Ali Rowhani-Rahbar; Frances B Alvarez; Janine T Bryan; James P Hughes; Stephen E Hawes; Noel S Weiss; Laura A Koutsky Journal: J Clin Virol Date: 2011-12-30 Impact factor: 3.168
Authors: Loghman Salimzadeh; Nina Le Bert; Charles-A Dutertre; Upkar S Gill; Evan W Newell; Christian Frey; Magdeleine Hung; Nikolai Novikov; Simon Fletcher; Patrick Tf Kennedy; Antonio Bertoletti Journal: J Clin Invest Date: 2018-08-07 Impact factor: 14.808
Authors: Ruth Simmons; Colin Sharp; Stuart Sims; Henrik Kloverpris; Philip Goulder; Peter Simmonds; Paul Bowness; Paul Klenerman Journal: J Infect Dis Date: 2011-05-15 Impact factor: 5.226
Authors: Alice R Burton; Laura J Pallett; Laura E McCoy; Kornelija Suveizdyte; Oliver E Amin; Leo Swadling; Elena Alberts; Brian R Davidson; Patrick Tf Kennedy; Upkar S Gill; Claudia Mauri; Paul A Blair; Nadege Pelletier; Mala K Maini Journal: J Clin Invest Date: 2018-08-09 Impact factor: 19.456