Nian-Kai Zeng1, Qiu-Ying Wang, Ming-Sheng Su. 1. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100094, China. niankaiz@163.com
Abstract
OBJECTIVE: To breed good strains by protoplast fusion in Phellinus baumii. METHODS: 5.8S rDNA-ITS sequences, mycelial growth rate, mycelial biomass and exopolysaccharides (EPS) production of 7 strains in different origins were analyzed, respectively. Then the inactivated parent protoplast and the PEG induction were used in the process of protoplast fusion. RESULTS: The strain P. baumii SA02 and P. baumii SA04 were selected as the parent strains. R002, R003 and R005 had antagonistic effects with parent strains. CONCLUSION: R002, R003 and R005 are fusants of the parent strains.
OBJECTIVE: To breed good strains by protoplast fusion in Phellinus baumii. METHODS: 5.8S rDNA-ITS sequences, mycelial growth rate, mycelial biomass and exopolysaccharides (EPS) production of 7 strains in different origins were analyzed, respectively. Then the inactivated parent protoplast and the PEG induction were used in the process of protoplast fusion. RESULTS: The strain P. baumii SA02 and P. baumii SA04 were selected as the parent strains. R002, R003 and R005 had antagonistic effects with parent strains. CONCLUSION: R002, R003 and R005 are fusants of the parent strains.