| Literature DB >> 18652847 |
Nadine Sadon1, Anne Delers, Richard G Jarman, Chonticha Klungthong, Ananda Nisalak, Robert V Gibbons, Ventzislav Vassilev.
Abstract
In order to detect and identify dengue serotypes in serum samples, we developed a single-step quantitative reverse transcription-polymerase chain reaction (Q-RT-PCR) assay (referred to as Q-PCR). Sets of primers were selected from the capsid region of the viral genome. Dengue serotypes 1/3 and 2/4 were detected in two separate duplex amplification reactions using specific primers and fluorogenic TaqMan probes. Results obtained with this Q-PCR and the classical nested RT-PCR (N-PCR) assays were compared using a panel of 97 representative human sera collected from patients in Bangkok, Thailand. It is shown that the Q-PCR is a rapid, sensitive and reproducible tool for the detection and quantitation of the four dengue serotypes in clinical samples, and therefore of great interest for diagnostic use or for large cohort studies.Entities:
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Year: 2008 PMID： 18652847 DOI： 10.1016/j.jviromet.2008.06.023
Source DB: PubMed Journal: J Virol Methods ISSN： 0166-0934 Impact factor: 2.014