Literature DB >> 18651069

On-site manipulation of single chromosomal DNA molecules by using optically driven microstructures.

Kyohei Terao1, Masao Washizu, Hidehiro Oana.   

Abstract

We report a novel method for manipulation of single giant DNA molecules under a video microscope. Using optically driven microstructures, we manipulated chromosomal DNA of length in the order of millimetres, extended by electroosmotic flow without DNA breakage in aqueous solution: we picked up DNA, using microfabricated hooks and wound it around microfabricated bobbins.

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Year:  2008        PMID： 18651069     DOI： 10.1039/b803753a

Source DB:  PubMed          Journal:  Lab Chip        ISSN： 1473-0189            Impact factor:   6.799


  4 in total

1.  Molecular ring toss of circular BAC DNA using micropillar array for single-molecule studies.

Authors:  Daiki Dohi; Ken Hirano; Kyohei Terao
Journal:  Biomicrofluidics       Date:  2020-02-21       Impact factor: 2.800

Review 2.  Microfluidic epigenomic mapping technologies for precision medicine.

Authors:  Chengyu Deng; Lynette B Naler; Chang Lu
Journal:  Lab Chip       Date:  2019-07-24       Impact factor: 6.799

3.  Characterisation of optically driven microstructures for manipulating single DNA molecules under a fluorescence microscope.

Authors:  Kyohei Terao; Chihiro Masuda; Ryo Inukai; Murat Gel; Hidehiro Oana; Masao Washizu; Takaaki Suzuki; Hidekuni Takao; Fusao Shimokawa; Fumikazu Oohira
Journal:  IET Nanobiotechnol       Date:  2016-06       Impact factor: 1.847

4.  On-site processing of single chromosomal DNA molecules using optically driven microtools on a microfluidic workbench.

Authors:  Akihito Masuda; Hidekuni Takao; Fusao Shimokawa; Kyohei Terao
Journal:  Sci Rep       Date:  2021-04-12       Impact factor: 4.379
  4 in total

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