Literature DB >> 1865054

Differential precipitation and zinc chelate chromatography purification of biologically active HTLV-I Tax1 expressed in E. coli.

P F Lindholm1, S J Marriott, S D Gitlin, J N Brady.   

Abstract

A protocol which involves sequential ammonium sulfate precipitation and zinc chelate chromatography to purify the HTLV-I Tax1 protein expressed in E. coli is described. The final Tax1 product is greater than 90% pure and the yield is approximately 1 mg per liter of liquid culture. The purified Tax1 protein is biologically active in indirect in vitro DNA binding assays and cellular NF-kB induction experiments.

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Year:  1991        PMID： 1865054     DOI： 10.1016/0165-022x(91)90071-4

Source DB:  PubMed          Journal:  J Biochem Biophys Methods        ISSN： 0165-022X


  6 in total

1.  Human T-cell leukemia virus type 1 Tax enhances serum response factor DNA binding and alters site selection.

Authors:  Heather Y Winter; Susan J Marriott
Journal:  J Virol       Date:  2007-03-21       Impact factor: 5.103

2.  Recombinant human T-cell leukemia virus types 1 and 2 Tax proteins induce high levels of CC-chemokines and downregulate CCR5 in human peripheral blood mononuclear cells.

Authors:  Christy S Barrios; Muna Abuerreish; Michael D Lairmore; Laura Castillo; Chou-Zen Giam; Mark A Beilke
Journal:  Viral Immunol       Date:  2011-11-23       Impact factor: 2.257

3.  Transcriptional up-regulation of the cyclin D2 gene and acquisition of new cyclin-dependent kinase partners in human T-cell leukemia virus type 1-infected cells.

Authors:  F Santiago; E Clark; S Chong; C Molina; F Mozafari; R Mahieux; M Fujii; N Azimi; F Kashanchi
Journal:  J Virol       Date:  1999-12       Impact factor: 5.103

4.  Human T-cell leukemia virus type 1 Tax transactivates the human proliferating cell nuclear antigen promoter.

Authors:  S Ressler; G F Morris; S J Marriott
Journal:  J Virol       Date:  1997-02       Impact factor: 5.103

5.  Extracellular Tax1 protein stimulates tumor necrosis factor-beta and immunoglobulin kappa light chain expression in lymphoid cells.

Authors:  P F Lindholm; R L Reid; J N Brady
Journal:  J Virol       Date:  1992-03       Impact factor: 5.103

6.  Transactivation of the human T-cell lymphotropic virus type 1 Tax1-responsive 21-base-pair repeats requires Holo-TFIID and TFIIA.

Authors:  J F Duvall; F Kashanchi; A Cvekl; M F Radonovich; G Piras; J N Brady
Journal:  J Virol       Date:  1995-08       Impact factor: 5.103
  6 in total

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