PURPOSE: The aim of this study was to measure the levels of oxidative DNA damage in cells isolated from the colon mucosa in patients with colorectal cancer and to compare normal and neoplastic tissues and make correlations with anatomopathologic variables. PATIENTS AND METHODS: Thirty-three patients with colorectal adenocarcinoma were studied. The oxidative DNA damage was evaluated by means of the alkaline version of the comet assay. RESULTS: For all the patients studied, it was found that the cells obtained from the neoplastic tissue presented oxidative DNA damage greater than in the cells from normal tissue. The cells isolated from the neoplastic mucosal tissue of the colon presented significantly greater mean extent of DNA strand breakage than the cells isolated from normal tissue. Additionally, the patients at earlier stages of the Dukes and TNM classifications presented higher levels of oxidative damage than those at more advanced stages. CONCLUSION: Assessment of the levels of oxidative damage at the different stages of colorectal carcinogenesis is of great interest because it enables evaluation of the effectiveness of antioxidant substances that could be used as preventive measures against the initial oxidative aggressive action on the colonic mucosa.
PURPOSE: The aim of this study was to measure the levels of oxidative DNA damage in cells isolated from the colon mucosa in patients with colorectal cancer and to compare normal and neoplastic tissues and make correlations with anatomopathologic variables. PATIENTS AND METHODS: Thirty-three patients with colorectal adenocarcinoma were studied. The oxidative DNA damage was evaluated by means of the alkaline version of the comet assay. RESULTS: For all the patients studied, it was found that the cells obtained from the neoplastic tissue presented oxidative DNA damage greater than in the cells from normal tissue. The cells isolated from the neoplastic mucosal tissue of the colon presented significantly greater mean extent of DNA strand breakage than the cells isolated from normal tissue. Additionally, the patients at earlier stages of the Dukes and TNM classifications presented higher levels of oxidative damage than those at more advanced stages. CONCLUSION: Assessment of the levels of oxidative damage at the different stages of colorectal carcinogenesis is of great interest because it enables evaluation of the effectiveness of antioxidant substances that could be used as preventive measures against the initial oxidative aggressive action on the colonic mucosa.
Authors: Caroline Caltabiano; Felipe Rodrigues Máximo; Ana Paula Pimentel Spadari; Daniel Duarte da Conceição Miranda; Marcia Milena Pivatto Serra; Marcelo Lima Ribeiro; Carlos Augusto Real Martinez Journal: Dig Dis Sci Date: 2010-11-02 Impact factor: 3.199
Authors: Anita C G Chua; Borut Klopcic; Ian C Lawrance; John K Olynyk; Debbie Trinder Journal: World J Gastroenterol Date: 2010-02-14 Impact factor: 5.742
Authors: Veronika Fedirko; Roberd M Bostick; Qi Long; W Dana Flanders; Marjorie L McCullough; Eduard Sidelnikov; Carrie R Daniel; Robin E Rutherford; Aasma Shaukat Journal: Cancer Epidemiol Biomarkers Prev Date: 2010-01 Impact factor: 4.254
Authors: Karina V Barros; Roberta A N Xavier; Gilclay G Abreu; Carlos A R Martinez; Marcelo L Ribeiro; Alessandra Gambero; Patrícia O Carvalho; Claudia M O Nascimento; Vera L F Silveira Journal: Lipids Health Dis Date: 2010-07-08 Impact factor: 3.876
Authors: Denise G Priolli; Thamy P Canelloi; Camila O Lopes; Júlio C M Valdívia; Natalia P Martinez; Demetrius P Açari; Izilda A Cardinalli; Marcelo L Ribeiro Journal: Int J Colorectal Dis Date: 2013-04-05 Impact factor: 2.571