Jing Rong1, Wei Chang, Ling Lv, Jun Chen. 1. Dept.of Occupational and Environmental Health, School of Public Health, Tongji Medical College of Huazhong University of Science and Technology, Key Laboratory of Environment and Health of Education Ministry of China, Wuhan 430030, China. rosej@163.com
Abstract
OBJECTIVE: To investigate the roles of nuclear factor-kappaB (NF-kappaB), p53 and Bcl-2 gene in lead acetate induced apoptosis in PC12 cells. METHODS: Cell culture, Flow cytometry, RT-PCR and Western Blot were employed to analyze the expression of NF-kappaB, p53 and Bcl-2 gene, and clarify the effect of lead acetate on apoptosis in PC12 cells and related molecular mechanisms. RESULTS: The PC12 cells were exposed to lead acetate at varied concentrations (100 micromol/ L, 200 micromol/L and 400 micromol/L) for 24 hours, Flow cytometry detected that the apoptosis rate of groups treated with lead acetate are higher than that of blank control groups with a dose-effect relationship (P < 0.05). RT-PCR results show that the mRNA expression level of NF-kappaB p65, p53 increased accordingly. Western Blot results show that the protein expression of Bcl-2 gene decreased. CONCLUSION: lead acetate induces apoptosis in PC12 cells, which may be related to activation of NF-kappaB and p53 gene and depression of Bcl-2 gene.
OBJECTIVE: To investigate the roles of nuclear factor-kappaB (NF-kappaB), p53 and Bcl-2 gene in lead acetate induced apoptosis in PC12 cells. METHODS: Cell culture, Flow cytometry, RT-PCR and Western Blot were employed to analyze the expression of NF-kappaB, p53 and Bcl-2 gene, and clarify the effect of lead acetate on apoptosis in PC12 cells and related molecular mechanisms. RESULTS: The PC12 cells were exposed to lead acetate at varied concentrations (100 micromol/ L, 200 micromol/L and 400 micromol/L) for 24 hours, Flow cytometry detected that the apoptosis rate of groups treated with lead acetate are higher than that of blank control groups with a dose-effect relationship (P < 0.05). RT-PCR results show that the mRNA expression level of NF-kappaB p65, p53 increased accordingly. Western Blot results show that the protein expression of Bcl-2 gene decreased. CONCLUSION:lead acetate induces apoptosis in PC12 cells, which may be related to activation of NF-kappaB and p53 gene and depression of Bcl-2 gene.
Authors: Mohamed A Dkhil; Mohamed S Al-Khalifa; Saleh Al-Quraishy; Rafat Zrieq; Ahmed Esmat Abdel Moneim Journal: Drug Des Devel Ther Date: 2016-06-02 Impact factor: 4.162