Yonghua Li1, Aiguo Ma, Xianning Shao, Zhen Du. 1. Department of Nutrition and Food Hygiene, Jining Medical College, Jining 272013, China. liyonghuaw@tom.com
Abstract
OBJECTIVE: To study the effect of antioxidant vitamin E (VE), vitamin C (VC) combined with different doses of beta-carotene (beta-C) supplementation on erythrocyte functions in the elderly person. METHODS:300 elderly person at the age from 60 to 75years old were randomized into 5 groups: from group 1 to group 4 were supplemented with (VC 300 + VE 200) mg/d and different doses of beta-C (16.7, 8.4, 5.6 and 0.0 mg/d, respectively), and control group 5 were supplemented with VE 5 mg/d. The trial lasted for 16 weeks. H2O2-caused hemolytic of erythrocytes was measured spectrometricalyl. ATPase activities in erythrocyte membrane were detected by colorimetric analysis. The fluidity of erythrocyte membrane was evaluated by fluorescence polarization (rho) and microviscosity (eta). All the data was analyzed with SPSS12.0. RESULTS:H2O2-caused hemolysis of erythrocyte was decreased significantly from group 1 to group 4 after the trial compared with before the trial (P < 0.01), they also had significant difference in compared with group 5 after the trial (P < 0.01). ATPase activities in erythrocyte membrane were more higher in group 1 than those of group 5 after the trial( P < 0.05). Moreover, rho and eta values were lower from 1 to 4 groups after the trial than itself before the trial (P < 0.01). CONCLUSION; Antioxidant VE, VC combined with different doses of beta-C supplementation could improve the function of erythrocyte in the elderly subjects.
RCT Entities:
OBJECTIVE: To study the effect of antioxidant vitamin E (VE), vitamin C (VC) combined with different doses of beta-carotene (beta-C) supplementation on erythrocyte functions in the elderly person. METHODS: 300 elderly person at the age from 60 to 75years old were randomized into 5 groups: from group 1 to group 4 were supplemented with (VC 300 + VE 200) mg/d and different doses of beta-C (16.7, 8.4, 5.6 and 0.0 mg/d, respectively), and control group 5 were supplemented with VE 5 mg/d. The trial lasted for 16 weeks. H2O2-caused hemolytic of erythrocytes was measured spectrometricalyl. ATPase activities in erythrocyte membrane were detected by colorimetric analysis. The fluidity of erythrocyte membrane was evaluated by fluorescence polarization (rho) and microviscosity (eta). All the data was analyzed with SPSS12.0. RESULTS:H2O2-caused hemolysis of erythrocyte was decreased significantly from group 1 to group 4 after the trial compared with before the trial (P < 0.01), they also had significant difference in compared with group 5 after the trial (P < 0.01). ATPase activities in erythrocyte membrane were more higher in group 1 than those of group 5 after the trial( P < 0.05). Moreover, rho and eta values were lower from 1 to 4 groups after the trial than itself before the trial (P < 0.01). CONCLUSION; Antioxidant VE, VC combined with different doses of beta-C supplementation could improve the function of erythrocyte in the elderly subjects.