| Literature DB >> 18641960 |
Natascha Kunert1, Alexander Brehm.
Abstract
The purification of native protein complexes requires the availability of sufficient amounts of starting material. Drosophila melanogaster embryos have proven to be a rich source for nuclear protein complexes. Here we describe establishment and maintenance of a fly facility for the production of large amounts of embryos, protocols for the production of nuclear extracts, and a scheme for the chromatographic purification of a nuclear multisubunit protein complex.Entities:
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Year: 2008 PMID： 18641960 DOI： 10.1007/978-1-59745-583-1_23
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745