Literature DB >> 18641953

Imaging Drosophila pupal wing morphogenesis.

Anne-Kathrin Classen1, Benoit Aigouy, Angela Giangrande, Suzanne Eaton.   

Abstract

Drosophila pupal (P) wing development entails a series of dynamic developmental events, such as epithelial and glial morphogenesis, that are of outstanding interest to cell biologists. Here, we first describe how to prepare P and prepupal (PP) wings for immunofluorescence microscopy. This protocol has been optimized to visualize wing epithelial architecture, such as polarized cortical domains of planar cell polarity proteins. We then provide a protocol to prepare pupae for whole mount live imaging of P wings. This procedure has allowed us to live-image glial cell migration and proliferation along wing sensory nerves.

Entities:  

Mesh：

Year:  2008        PMID： 18641953     DOI： 10.1007/978-1-59745-583-1_16

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  21 in total

Review 1.  Methods for studying planar cell polarity.

Authors:  Jessica Olofsson; Jeffrey D Axelrod
Journal:  Methods       Date:  2014-03-27       Impact factor: 3.608

2.  Live-imaging of the Drosophila pupal eye.

Authors:  Mark B Hellerman; Richard H Choe; Ruth I Johnson
Journal:  J Vis Exp       Date:  2015-01-12       Impact factor: 1.355

Review 3.  Imaging morphogenesis.

Authors:  Donald M Bell
Journal:  Philos Trans R Soc Lond B Biol Sci       Date:  2017-05-19       Impact factor: 6.237

Review 4.  Cargo sorting in the endocytic pathway: a key regulator of cell polarity and tissue dynamics.

Authors:  Suzanne Eaton; Fernando Martin-Belmonte
Journal:  Cold Spring Harb Perspect Biol       Date:  2014-08-14       Impact factor: 10.005

5.  A Rapid and Efficient Method to Dissect Pupal Wings of Drosophila Suitable for Immunodetections or PCR Assays.

Authors:  Carmen Bolatto; Cristina Parada; Victoria Colmenares
Journal:  J Vis Exp       Date:  2017-12-30       Impact factor: 1.355

6.  Interplay of cell dynamics and epithelial tension during morphogenesis of the Drosophila pupal wing.

Authors:  Raphaël Etournay; Marko Popović; Matthias Merkel; Amitabha Nandi; Corinna Blasse; Benoît Aigouy; Holger Brandl; Gene Myers; Guillaume Salbreux; Frank Jülicher; Suzanne Eaton
Journal:  Elife       Date:  2015-06-23       Impact factor: 8.140

7.  The NDNF-like factor Nord is a Hedgehog-induced extracellular BMP modulator that regulates Drosophila wing patterning and growth.

Authors:  Shu Yang; Xuefeng Wu; Euphrosyne I Daoutidou; Ya Zhang; MaryJane Shimell; Kun-Han Chuang; Aidan J Peterson; Michael B O'Connor; Xiaoyan Zheng
Journal:  Elife       Date:  2022-01-17       Impact factor: 8.140

8.  Use of Fluorescence Recovery After Photobleaching (FRAP) to Measure In Vivo Dynamics of Cell Junction-Associated Polarity Proteins.

Authors:  Samantha J Warrington; Helen Strutt; David Strutt
Journal:  Methods Mol Biol       Date:  2022

9.  Unified quantitative characterization of epithelial tissue development.

Authors:  Boris Guirao; Stéphane U Rigaud; Floris Bosveld; Anaïs Bailles; Jesús López-Gay; Shuji Ishihara; Kaoru Sugimura; François Graner; Yohanns Bellaïche
Journal:  Elife       Date:  2015-12-12       Impact factor: 8.140

10.  Combinatorial control of temporal gene expression in the Drosophila wing by enhancers and core promoters.

Authors:  David D O'Keefe; Sean R Thomas; Kelsey Bolin; Ellen Griggs; Bruce A Edgar; Laura A Buttitta
Journal:  BMC Genomics       Date:  2012-09-20       Impact factor: 3.969
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