Literature DB >> 18641952

Immunolabeling of imaginal discs.

Thomas Klein1.   

Abstract

Drosophila melanogaster imaginal discs are a widely used model system to study signal transduction, developmental, and cell biological processes. An important tool to study these processes is immunolabeling. Fluorochrome-conjugated antibody staining allows the researcher to determine the expression pattern and subcellular localization of several proteins in one imaginal disc. In this chapter, I will describe how to dissect, fix, and immunolabel imaginal discs. The protocol is streamlined so that the whole procedure can be performed in 1 d, including image acquisition.

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Year:  2008        PMID： 18641952     DOI： 10.1007/978-1-59745-583-1_15

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  10 in total

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3.  A population of G2-arrested cells are selected as sensory organ precursors for the interommatidial bristles of the Drosophila eye.

Authors:  Joy H Meserve; Robert J Duronio
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4.  Fate mapping during regeneration: Cells that undergo compensatory proliferation in damaged Drosophila eye imaginal discs differentiate into multiple retinal accessory cell types.

Authors:  Joy H Meserve; Robert J Duronio
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5.  An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.

Authors:  Daiki Umetsu; Sebastian Dunst; Christian Dahmann
Journal:  PLoS One       Date:  2014-12-04       Impact factor: 3.240

6.  Dynamic genome wide expression profiling of Drosophila head development reveals a novel role of Hunchback in retinal glia cell development and blood-brain barrier integrity.

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7.  Differential lateral and basal tension drive folding of Drosophila wing discs through two distinct mechanisms.

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8.  Lethal (2) giant discs (Lgd)/CC2D1 is required for the full activity of the ESCRT machinery.

Authors:  Miriam Baeumers; Kristina Ruhnau; Thomas Breuer; Hendrik Pannen; Bastian Goerlich; Anna Kniebel; Sebastian Haensch; Stefanie Weidtkamp-Peters; Lutz Schmitt; Thomas Klein
Journal:  BMC Biol       Date:  2020-12-22       Impact factor: 7.431

9.  Preparation of Precisely Oriented Cryosections of Undistorted Drosophila Wing Imaginal Discs for High Resolution Confocal Imaging.

Authors:  Samuel Petshow; Marcel Wehrli
Journal:  Bio Protoc       Date:  2018-02-05

10.  Tools and methods for studying Notch signaling in Drosophila melanogaster.

Authors:  Evanthia Zacharioudaki; Sarah J Bray
Journal:  Methods       Date:  2014-04-03       Impact factor: 3.608
  10 in total

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