Jianxiong Yang1, Yali Wang, Yu Bao, Juan Guo. 1. College of Life Science, Shaanxi Normal University, Xi'an 710062, Shaanxi, China. jxyang@snnu.edu.cn
Abstract
OBJECTIVE: To discover the effect of the total flavones from Semen cuscutae (TFSC) on the kidney-yang deficiency male mouse, especially on hormone levels and the androgen receptor (AR) mRNA and protein level in the kidney and testicle. METHODS: ICR male mouse were separated into six groups, and, except for the normal group, hydrocortisone was injected intraperitoneally to make the kidney-yang deficiency. The groups were then treated with TFSC, methyltestosterone and Jinkui Shenqi Wan, except for the normal group and control group. 14 days after administering, testosterone levels in the total serum were analysed by radioimmunoassay, AR mRNA levels measured by real time RT-PCR and protein levels by immunohistochemistry. RESULTS: The control group had the lowest testosterone level, AR mRNA level and protein level, and the TFSC can reverse the reduction of testosterone level, AR mRNA level and protein level induced by the hydrocortisone. CONCLUSIONS: This is the first study to demonstrate that TFSC treatment may reverse kidney-yang deficiency symptoms by restoring the levels of testosterone and AR mRNA and protein expression in the kidney and testicle.
OBJECTIVE: To discover the effect of the total flavones from Semen cuscutae (TFSC) on the kidney-yang deficiency male mouse, especially on hormone levels and the androgen receptor (AR) mRNA and protein level in the kidney and testicle. METHODS: ICR male mouse were separated into six groups, and, except for the normal group, hydrocortisone was injected intraperitoneally to make the kidney-yang deficiency. The groups were then treated with TFSC, methyltestosterone and Jinkui Shenqi Wan, except for the normal group and control group. 14 days after administering, testosterone levels in the total serum were analysed by radioimmunoassay, AR mRNA levels measured by real time RT-PCR and protein levels by immunohistochemistry. RESULTS: The control group had the lowest testosterone level, AR mRNA level and protein level, and the TFSC can reverse the reduction of testosterone level, AR mRNA level and protein level induced by the hydrocortisone. CONCLUSIONS: This is the first study to demonstrate that TFSC treatment may reverse kidney-yang deficiency symptoms by restoring the levels of testosterone and AR mRNA and protein expression in the kidney and testicle.
Authors: Kiran Kumar Soni; Li Tao Zhang; Jae Hyung You; Sung Won Lee; Chul Young Kim; Wan Shou Cui; Han Jung Chae; Hye Kyung Kim; Jong Kwan Park Journal: Cancer Cell Int Date: 2015-12-18 Impact factor: 5.722