Nucleotide sequence of the polyhedrin gene of Autographa californica nuclear polyhedrosis virus.

The nucleotide sequence of a 1143-bp region of Autographa californica MNPV (AcMNPV) DNA containing the entire coding region of polyhedrin was determined. The coding region consisted of 732 bp without intervening sequences. The 5' leader sequence was approximately 58 nucleotides in length. A putative "TATA box" and "CAAT box" were located 20 and 52 bp upstream from the transcriptional start site, respectively. Two tandem repeats of 8 bp were found 70 and 84 bp upstream from the transcriptional start site. The molecular weight of polyhedrin, as deduced from the nucleotide sequence, was 28,872. The amino acid sequence of AcMNPV polyhedrin was very similar to the amino acid sequence of Bombyx mori polyhedrin.