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Literature DB >> 18638485

Rapid kinetic analysis of transcription elongation by Escherichia coli RNA polymerase.

Ronald S Johnson¹, Mark Strausbauch, Ryan Cooper, J Kristen Register.

Abstract

Nucleotide incorporation during transcription by RNA polymerase is accompanied by pyrophosphate formation. Rapid release of pyrophosphate from the elongation complex at a rate consistent with productive transcription elongation occurs only in the presence of the correct next nucleotide for incorporation into the transcript.

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Year: 2008 PMID： 18638485 DOI： 10.1016/j.jmb.2008.06.089

Source DB: PubMed Journal: J Mol Biol ISSN： 0022-2836 Impact factor: 5.469

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Cited

16 in total

1. Trigger loop dynamics mediate the balance between the transcriptional fidelity and speed of RNA polymerase II.

Authors: Matthew H Larson; Jing Zhou; Craig D Kaplan; Murali Palangat; Roger D Kornberg; Robert Landick; Steven M Block
Journal: Proc Natl Acad Sci U S A Date: 2012-04-09 Impact factor: 11.205

2. Conformational coupling, bridge helix dynamics and active site dehydration in catalysis by RNA polymerase.

Authors: Steve A Seibold; Badri Nath Singh; Chunfen Zhang; Maria Kireeva; Céline Domecq; Annie Bouchard; Anthony M Nazione; Michael Feig; Robert I Cukier; Benoit Coulombe; Mikhail Kashlev; Michael Hampsey; Zachary F Burton
Journal: Biochim Biophys Acta Date: 2010-05-15

Rapid kinetic analysis of transcription elongation by Escherichia coli RNA polymerase.

1. Trigger loop dynamics mediate the balance between the transcriptional fidelity and speed of RNA polymerase II.

2. Conformational coupling, bridge helix dynamics and active site dehydration in catalysis by RNA polymerase.

3. Development of a "modular" scheme to describe the kinetics of transcript elongation by RNA polymerase.

Review 4. The Mechanisms of Substrate Selection, Catalysis, and Translocation by the Elongating RNA Polymerase.

5. Templated nucleoside triphosphate binding to a noncatalytic site on RNA polymerase regulates transcription.

6. Intrinsic translocation barrier as an initial step in pausing by RNA polymerase II.

7. The RNA polymerase bridge helix YFI motif in catalysis, fidelity and translocation.

8. A quantitative stopped-flow fluorescence assay for measuring polymerase elongation rates.

9. Millisecond phase kinetic analysis of elongation catalyzed by human, yeast, and Escherichia coli RNA polymerase.

Review 10. Single-molecule studies of RNAPII elongation.