OBJECTIVE: In this study we developed a 96-micro plate enzymatic assay for D-lactate in plasma and urine. METHODS: D-lactate was assayed enzymatically with a UV-spectrophotometer in plasma from 38 and in urine from 37 diabetics and from 42 non-diabetic controls. RESULTS: The within-run coefficients of variations (CV) were 2.6% for plasma and 5.7% for urine. The between run CVs were 6.8% for plasma and 6.7% for urine. The mean recovery with standard deviation (S.D.) was 107.4+/-7.3% for plasma and 100.1%+/-6.7% for urine. The plasma D-lactate in diabetics were (mean+/-S.D.) 39.6+/-23.7 microM. We found significant difference between the urinary d-lactate in controls and diabetics (18.2+/-12.0 vs. 35.9+/-24.2 microM/mM creatinine, p<0.0001). CONCLUSIONS: The assay proved reliable with acceptable precision and recovery. Results suggest that diabetics have elevated urinary and plasma D-lactate as compared to controls.
OBJECTIVE: In this study we developed a 96-micro plate enzymatic assay for D-lactate in plasma and urine. METHODS:D-lactate was assayed enzymatically with a UV-spectrophotometer in plasma from 38 and in urine from 37 diabetics and from 42 non-diabetic controls. RESULTS: The within-run coefficients of variations (CV) were 2.6% for plasma and 5.7% for urine. The between run CVs were 6.8% for plasma and 6.7% for urine. The mean recovery with standard deviation (S.D.) was 107.4+/-7.3% for plasma and 100.1%+/-6.7% for urine. The plasma D-lactate in diabetics were (mean+/-S.D.) 39.6+/-23.7 microM. We found significant difference between the urinary d-lactate in controls and diabetics (18.2+/-12.0 vs. 35.9+/-24.2 microM/mM creatinine, p<0.0001). CONCLUSIONS: The assay proved reliable with acceptable precision and recovery. Results suggest that diabetics have elevated urinary and plasma D-lactate as compared to controls.
Authors: Jean L J M Scheijen; Nordin M J Hanssen; Marjo P H van de Waarenburg; Daisy M A E Jonkers; Coen D A Stehouwer; Casper G Schalkwijk Journal: Exp Diabetes Res Date: 2012-03-08
Authors: Anna Ka-Yee Kwong; Sheila Suet-Na Wong; Richard J T Rodenburg; Jan Smeitink; Godfrey Chi Fung Chan; Cheuk-Wing Fung Journal: JIMD Rep Date: 2021-05-21