| Literature DB >> 18635002 |
Yuki Kinjo1, Bo Pei, Simone Bufali, Ravinder Raju, Stewart K Richardson, Masakazu Imamura, Masakazu Fujio, Douglass Wu, Archana Khurana, Kazuyoshi Kawahara, Chi-Huey Wong, Amy R Howell, Peter H Seeberger, Mitchell Kronenberg.
Abstract
Mouse natural killer T (NKT) cells expressing an invariant T cell antigen receptor (TCR) recognize glycosphingolipids (GSLs) from Sphingomonas bacteria. The synthetic antigens previously tested, however, were designed to closely resemble the potent synthetic agonist alpha-galactosyl ceramide (alphaGalCer), which contains a monosaccharide and a C18:0 sphingosine lipid. Some Sphingomonas bacteria, however, also have oligosaccharide-containing GSLs, and they normally synthesize several GSLs with different sphingosine chains including one with a cyclopropyl ring-containing C21:0 (C21cycl) sphingosine. Here we studied the stimulation of NKT cells with synthetic GSL antigens containing natural tetrasaccharide sugars, or the C21cycl sphingosine. Our results indicate that there is a great degree of variability in the antigenic potency of different natural Sphingomonas glycolipids, with the C21cycl sphingosine having intermediate potency and the oligosaccharide-containing antigens exhibiting limited or no stimulatory capacity.Entities:
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Year: 2008 PMID： 18635002 PMCID： PMC2519143 DOI： 10.1016/j.chembiol.2008.05.012
Source DB: PubMed Journal: Chem Biol ISSN： 1074-5521