OBJECTIVES: To evaluate the intracellular levels of the high energy adenosine triphosphate nucleotide ATP and essential divalent cations, calcium and magnesium, in platelets of patients affected by primary fibromyalgia syndrome (FMs). DESIGN AND METHOD: Platelet ATP and cation concentrations were measured in 25 patients affected by FMs and 25 healthy volunteers through a chemiluminescent and a fluorimetric assay, respectively. RESULTS: Significant lower ATP levels were observed inside platelets of FM patients (fmol ATP/plt: 0.0169+/-0.0012 vs. healthy controls, fmol ATP/plt: 0.0306+/-0.0023, mean+/-SEM) (*** P<0.0001). A trend towards higher calcium concentrations (P=0.06) together with significant increased magnesium levels were also reported in platelets of patients by comparison with controls (P=0.02). CONCLUSIONS: This preliminary study suggests that disturbances in the homeostasis of platelet ATP metabolism-signaling and calcium-magnesium flows might have a relevance in the pathogenesis of FMs.
OBJECTIVES: To evaluate the intracellular levels of the high energy adenosine triphosphate nucleotideATP and essential divalent cations, calcium and magnesium, in platelets of patients affected by primary fibromyalgia syndrome (FMs). DESIGN AND METHOD: Platelet ATP and cation concentrations were measured in 25 patients affected by FMs and 25 healthy volunteers through a chemiluminescent and a fluorimetric assay, respectively. RESULTS: Significant lower ATP levels were observed inside platelets of FM patients (fmol ATP/plt: 0.0169+/-0.0012 vs. healthy controls, fmol ATP/plt: 0.0306+/-0.0023, mean+/-SEM) (*** P<0.0001). A trend towards higher calcium concentrations (P=0.06) together with significant increased magnesium levels were also reported in platelets of patients by comparison with controls (P=0.02). CONCLUSIONS: This preliminary study suggests that disturbances in the homeostasis of platelet ATP metabolism-signaling and calcium-magnesium flows might have a relevance in the pathogenesis of FMs.
Authors: Pedro Bullón; Elísabet Alcocer-Gómez; Angel M Carrión; Fabiola Marín-Aguilar; Juan Garrido-Maraver; Lourdes Román-Malo; Jesus Ruiz-Cabello; Ognjen Culic; Bernhard Ryffel; Lionel Apetoh; François Ghiringhelli; Maurizio Battino; José Antonio Sánchez-Alcazar; Mario D Cordero Journal: Antioxid Redox Signal Date: 2015-09-16 Impact factor: 8.401
Authors: L Bazzichi; A Rossi; C Zirafa; F Monzani; S Tognini; A Dardano; F Santini; M Tonacchera; M De Servi; C Giacomelli; F De Feo; M Doveri; G Massimetti; S Bombardieri Journal: Rheumatol Int Date: 2010-11-18 Impact factor: 2.631
Authors: Ruth E McDowell; Kulwant S Aulak; Allaa Almoushref; Celia A Melillo; Brittany E Brauer; Jennie E Newman; Adriano R Tonelli; Raed A Dweik Journal: Am J Physiol Lung Cell Mol Physiol Date: 2020-02-05 Impact factor: 6.011
Authors: Monica L Joustra; Isidor Minovic; Karin A M Janssens; Stephan J L Bakker; Judith G M Rosmalen Journal: PLoS One Date: 2017-04-28 Impact factor: 3.240
Authors: Mario D Cordero; Manuel De Miguel; Ana M Moreno Fernández; Inés M Carmona López; Juan Garrido Maraver; David Cotán; Lourdes Gómez Izquierdo; Pablo Bonal; Francisco Campa; Pedro Bullon; Plácido Navas; José A Sánchez Alcázar Journal: Arthritis Res Ther Date: 2010-01-28 Impact factor: 5.156