Literature DB >> 1863352

Enumeration of antigen sites on cells by flow cytometry.

C D Dawson1, J A Scheppler, J K Nicholson, R C Holman.   

Abstract

We evaluated a cytofluorometric method for determining the number of antigens expressed on the cell surface of human lymphocytes. Using beads that have a known number of binding sites for mouse immunoglobulin and monoclonal antibodies specific for various antigens on human lymphocytes, we found that this system is quite reproducible, reliable and technically easy to perform. The greatest source of variation in expression of cell surface antigens is interdonor variability.

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Year:  1991        PMID： 1863352     DOI： 10.1159/000163617

Source DB:  PubMed          Journal:  Pathobiology        ISSN： 1015-2008            Impact factor:   4.342


  3 in total

1.  Granulocyte colony-stimulating factor improves deficient in vitro neutrophil transendothelial migration in patients with advanced liver disease.

Authors:  Carmen Fiuza; Magdalena Salcedo; Gerardo Clemente; Jose M Tellado
Journal:  Clin Diagn Lab Immunol       Date:  2002-03

2.  The relationship between flow-cytometric and immunohistochemically detected c-erbB-2 expression, grade and DNA ploidy in breast cancer.

Authors:  I Brotherick; B K Shenton; W K Cowan; B Angus; C H Horne; M J Higgs; T W Lennard
Journal:  Cancer Immunol Immunother       Date:  1995-09       Impact factor: 6.968

3.  Effective use of frozen donor peripheral blood mononuclear cells for human immunodeficiency virus type 1 isolation from vertically infected pediatric patients.

Authors:  M O Paul; S Tetali; S Pahwa
Journal:  J Clin Microbiol       Date:  1994-05       Impact factor: 5.948
  3 in total

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