Literature DB >> 18631639

Transfection with baculovirus DNA.

J P Burand1, M D Summers, G E Smith.   

Abstract

Purified DNA from the nuclear polyhedrosis viruses of Autographa californica (AcM NPV) and Rachiplusia ou (RoMNPV) were found to be infectious in TN-368 cells employing the calcium phosphate precipitation technique (F. L. Graham and A. J. van der Eb, Virology, 52 ,456-467, 1973). Transfection with AcM NPV produced 3600 PFU/microg DNA compared to 2900 PFU/mug DNA with RoM NPV. Of eight baculovirus DNAs tested, only AcM NPV DNA and RoM NPV DNA could transfect TN-368 cells. The in vitro host range of AcM NPV DNA was determined to be the same as AcM NPV extracellular virus. AcM NPV Form I DNA was fourfold more infectious in TN-368 cells than Form II DNA.

Entities:  

Year:  1980        PMID： 18631639     DOI： 10.1016/0042-6822(80)90505-x

Source DB:  PubMed          Journal:  Virology        ISSN： 0042-6822            Impact factor:   3.616


  5 in total

1.  Replication patterns and cytopathology of cells infected with baculoviruses.

Authors:  G V Williams; P Faulkner
Journal:  Cytotechnology       Date:  1996-01       Impact factor: 2.058

Review 2.  Expression of eukaryotic genes in insect cultures.

Authors:  M J Fraser
Journal:  In Vitro Cell Dev Biol       Date:  1989-03

3.  Transfection techniques for producing recombinant baculoviruses.

Authors:  K M Trotter; H A Wood
Journal:  Mol Biotechnol       Date:  1996-12       Impact factor: 2.695

4.  Expression of the X protein of hepatitis B virus in insect cells using recombinant baculoviruses.

Authors:  R Klein; C H Schröder; H Zentgraf
Journal:  Virus Genes       Date:  1991-04       Impact factor: 2.332

5.  Multiplication of a granulosis virus isolated from the potato tuber moth in a new established cell line of Phthorimaea operculella.

Authors:  X Léry; J Giannotti; A Taha; M Ravalec; S Abol-Ela
Journal:  In Vitro Cell Dev Biol Anim       Date:  1997-09       Impact factor: 2.416
  5 in total

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