Literature DB >> 18631508

Rapid fixation and embedding for electron microscopy.

M A Hayat1, R Giaquinta.   

Abstract

A method has been developed for rapid processing of animal tissues for electron microscopy. The whole process of fixation staining dehydration, infiltration and embedding including polymerization is completed in less than 4 hr. A variety of human and animal tissues such as liver, spleen, muscle, kidney and embryonic chick heart were processed by this method and the results were excellent. The rapid fixation and embedding method is strongly recommended when relatively soft tissues are to be studied. This method is especially useful for examining pathological tissues for rapid diagnostic purposes.

Entities:  

Year:  1970        PMID： 18631508     DOI： 10.1016/s0040-8166(70)80015-5

Source DB:  PubMed          Journal:  Tissue Cell        ISSN： 0040-8166            Impact factor:   2.466


  10 in total

1.  Rapid specimen preparation to improve the throughput of electron microscopic volume imaging for three-dimensional analyses of subcellular ultrastructures with serial block-face scanning electron microscopy.

Authors:  Truc Quynh Thai; Huy Bang Nguyen; Sei Saitoh; Bao Wu; Yurika Saitoh; Satoshi Shimo; Yaser Hosny Ali Elewa; Osamu Ichii; Yasuhiro Kon; Takashi Takaki; Kensuke Joh; Nobuhiko Ohno
Journal:  Med Mol Morphol       Date:  2016-02-11       Impact factor: 2.309

2.  Rigid Embedding of Fixed and Stained, Whole, Millimeter-Scale Specimens for Section-free 3D Histology by Micro-Computed Tomography.

Authors:  Alex Y Lin; Yifu Ding; Daniel J Vanselow; Spencer R Katz; Maksim A Yakovlev; Darin P Clark; David Mandrell; Jean E Copper; Damian B van Rossum; Keith C Cheng
Journal:  J Vis Exp       Date:  2018-10-17       Impact factor: 1.355

Review 3.  Out with the old and in with the new: rapid specimen preparation procedures for electron microscopy of sectioned biological material.

Authors:  Kent L McDonald
Journal:  Protoplasma       Date:  2013-11-21       Impact factor: 3.356

4.  The fine structure of Micrococcus cyaneus.

Authors:  M T Silva; M Kocur
Journal:  Arch Mikrobiol       Date:  1972

5.  The acrosome reaction in the sperm of the shipworm Bankia australis Calman (Bivalvia, Mollusca).

Authors:  J D Popham
Journal:  Cell Tissue Res       Date:  1974       Impact factor: 5.249

6.  [Freeze ultramicrotomy of bone marrow].

Authors:  E Morgenstern; G Werner; K Neumann; D Hufnagl
Journal:  Blut       Date:  1973-04

7.  Morphological/chemical imaging of demineralized dentin layer in its natural, wet state.

Authors:  Yong Wang; Xiaomei Yao
Journal:  Dent Mater       Date:  2010-02-06       Impact factor: 5.304

8.  Quantitatively Understanding Cellular Uptake of Gold Nanoparticles via Radioactivity Analysis.

Authors:  Xia Shao; Paul Schnau; Wei Qian; Xueding Wang
Journal:  J Nanosci Nanotechnol       Date:  2015-05

9.  A Workflow for High-pressure Freezing and Freeze Substitution of the Caenorhabditis elegans Embryo for Ultrastructural Analysis by Conventional and Volume Electron Microscopy.

Authors:  Mohammad M Rahman; Irene Y Chang; Orna Cohen-Fix; Kedar Narayan
Journal:  Bio Protoc       Date:  2021-04-05

10.  Detection and identification of viruses by electron microscopy.

Authors:  Sara E Miller
Journal:  J Electron Microsc Tech       Date:  2005-02-04
  10 in total

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