K Papasouliotis1, S Dodkin, K F Murphy, A Sladen. 1. Langford Veterinary Diagnostics, School of Clinical Veterinary Science, University of Bristol, Langford, Bristol BS40 5DU, UK.
Abstract
OBJECTIVES: Falcor 350 (A. Menarini Diagnostics) is a wet-reagent biochemistry analyser that is available for in-house use. The aim of this study was to compare the results produced by this analyser with those obtained by a wet-reagent analyser (KoneLab 30i; Thermo Clinical Labsystems) that served as the reference instrument. METHODS: Blood samples from 120 clinical cases (60 dogs and 60 cats) were analysed for 18 analytes (urea, creatinine, total proteins, albumin, creatine kinase, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, total bilirubin, amylase, lipase, glucose, triacylglycerol, cholesterol, total calcium, phosphate, sodium and potassium) using both the reference and Falcor instruments. RESULTS: Good to excellent correlations (r(s) value) (canine/feline) were identified for urea (0.87/0.86), creatinine (0.96/0.99), total proteins (0.91/0.95), albumin (0.96/0.93), creatine kinase (0.98/0.99), aspartate aminotransferase (0.95/0.98), alanine aminotransferase (0.99/0.99), alkaline phosphatase (0.99/0.98), total bilirubin in dogs (0.88), amylase (0.99/0.87), lipase in dogs (0.88), glucose (0.97/0.98), triacylglycerol (0.93/0.97), cholesterol (0.99/0.99), total calcium (0.88/0.89), phosphate (0.94/0.97) and potassium (0.96/0.97). The correlations for sodium (0.41/0.61), total bilirubin in cats (0.78) and lipase in cats (0.25) were considered unacceptable. CLINICAL SIGNIFICANCE: For 13 of the 18 analytes (creatinine, total proteins, albumin, creatine kinase, aspartate aminotransferase, alanine aminotransferase, amylase, glucose, cholesterol, triacylglycerol, phosphate, potassium and urea) in both canine and feline samples, the two instruments produce values that are closely related to each other (excellent correlation) and are sufficiently similar to allow them to be used interchangeably without the need for additional correction factor computations (good agreement). Because of differences in the methodologies, the Falcor results for alkaline phosphatase, total calcium, sodium, lipase and total bilirubin cannot be used interchangeably with those generated by the KoneLab and should be interpreted using reference intervals established from the Falcor analyser.
OBJECTIVES: Falcor 350 (A. Menarini Diagnostics) is a wet-reagent biochemistry analyser that is available for in-house use. The aim of this study was to compare the results produced by this analyser with those obtained by a wet-reagent analyser (KoneLab 30i; Thermo Clinical Labsystems) that served as the reference instrument. METHODS: Blood samples from 120 clinical cases (60 dogs and 60 cats) were analysed for 18 analytes (urea, creatinine, total proteins, albumin, creatine kinase, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, total bilirubin, amylase, lipase, glucose, triacylglycerol, cholesterol, total calcium, phosphate, sodium and potassium) using both the reference and Falcor instruments. RESULTS: Good to excellent correlations (r(s) value) (canine/feline) were identified for urea (0.87/0.86), creatinine (0.96/0.99), total proteins (0.91/0.95), albumin (0.96/0.93), creatine kinase (0.98/0.99), aspartate aminotransferase (0.95/0.98), alanine aminotransferase (0.99/0.99), alkaline phosphatase (0.99/0.98), total bilirubin in dogs (0.88), amylase (0.99/0.87), lipase in dogs (0.88), glucose (0.97/0.98), triacylglycerol (0.93/0.97), cholesterol (0.99/0.99), total calcium (0.88/0.89), phosphate (0.94/0.97) and potassium (0.96/0.97). The correlations for sodium (0.41/0.61), total bilirubin in cats (0.78) and lipase in cats (0.25) were considered unacceptable. CLINICAL SIGNIFICANCE: For 13 of the 18 analytes (creatinine, total proteins, albumin, creatine kinase, aspartate aminotransferase, alanine aminotransferase, amylase, glucose, cholesterol, triacylglycerol, phosphate, potassium and urea) in both canine and feline samples, the two instruments produce values that are closely related to each other (excellent correlation) and are sufficiently similar to allow them to be used interchangeably without the need for additional correction factor computations (good agreement). Because of differences in the methodologies, the Falcor results for alkaline phosphatase, total calcium, sodium, lipase and total bilirubin cannot be used interchangeably with those generated by the KoneLab and should be interpreted using reference intervals established from the Falcor analyser.