BACKGROUND: Cell division cycle 20 homologue (CDC20), which encodes a protein that promotes chromosomal separation, is highly expressed in several carcinomas, including pancreatic cancer. MATERIALS AND METHODS: To ascertain whether this gene could be a potential therapeutic target, the RNA interference technique was applied using small interfering RNA (siRNA) to knockdown CDC20 expression. RESULTS: The CDC20 siRNA showed more than 90% inhibition of CDC20 expression at both the transcriptional and translational levels and the specific knockdown of CDC20 expression inhibited the cell growth of human pancreatic carcinoma cells in vitro. Suppression of CDC20 induced accumulation of the cells in the G2/M-phase of the cell cycle. In addition, the knockdown of CDC20 caused enhancement of the cytotoxicity of paclitaxel and increased the effect of gamma-irradiation against pancreatic carcinoma cells. CONCLUSION: CDC20 is a promising target for gene specific therapy in human pancreatic cancer.
BACKGROUND: Cell division cycle 20 homologue (CDC20), which encodes a protein that promotes chromosomal separation, is highly expressed in several carcinomas, including pancreatic cancer. MATERIALS AND METHODS: To ascertain whether this gene could be a potential therapeutic target, the RNA interference technique was applied using small interfering RNA (siRNA) to knockdown CDC20 expression. RESULTS: The CDC20 siRNA showed more than 90% inhibition of CDC20 expression at both the transcriptional and translational levels and the specific knockdown of CDC20 expression inhibited the cell growth of humanpancreatic carcinoma cells in vitro. Suppression of CDC20 induced accumulation of the cells in the G2/M-phase of the cell cycle. In addition, the knockdown of CDC20 caused enhancement of the cytotoxicity of paclitaxel and increased the effect of gamma-irradiation against pancreatic carcinoma cells. CONCLUSION:CDC20 is a promising target for gene specific therapy in humanpancreatic cancer.
Authors: H Karra; H Repo; I Ahonen; E Löyttyniemi; R Pitkänen; M Lintunen; T Kuopio; M Söderström; P Kronqvist Journal: Br J Cancer Date: 2014-05-22 Impact factor: 7.640
Authors: Susanne Lub; Anke Maes; Ken Maes; Kim De Veirman; Elke De Bruyne; Eline Menu; Karel Fostier; Alboukadel Kassambara; Jérôme Moreaux; Dirk Hose; Xavier Leleu; Randall W King; Karin Vanderkerken; Els Van Valckenborgh Journal: Oncotarget Date: 2016-01-26
Authors: Manoj B Parmar; Hamidreza Montazeri Aliabadi; Parvin Mahdipoor; Cezary Kucharski; Robert Maranchuk; Judith C Hugh; Hasan Uludağ Journal: Front Bioeng Biotechnol Date: 2015-02-16