BACKGROUND AND AIM: Glycine N-methyltransferase (GNMT) is a susceptibility gene for human hepatocellular carcinoma (HCC). We previously reported that GNMT expression is diminished in HCC. Here we report our examination of GNMT expression patterns in cholangiocarcinoma and the relationship between its expression and prognosis. METHODS: We analyzed GNMT expression in tumor tissues from 33 cholangiocarcinoma patients (19 male) using immunohistochemistry (IHC) procedures with a GNMT monoclonal antibody (mAb 4-17). GNMT expression intensity and percentages were scored on a scale of 0 to 6. The association between GNMT expression and survival was analyzed using the Kaplan-Meier method, and prognostic factors were evaluated with a multivariate Cox proportional hazards regression model. RESULTS: High GNMT expression was found in epithelial cells of normal bile ducts. Six of 33 (18.2%) cholangiocarcinoma tissues had no GNMT expression. A statistically significant difference was noted in GNMT expression between male and female patients (68.4% vs 100%, P < 0.05). Compared to patients with GNMT expression scores > 3, the death hazard ratio for patients with GNMT scores <or= 3 was 3.68 (95% confidence interval = 1.17-11.59, P < 0.05). CONCLUSIONS: GNMT expression is a favorable prognosis predictor for cholangiocarcinoma.
BACKGROUND AND AIM: Glycine N-methyltransferase (GNMT) is a susceptibility gene for humanhepatocellular carcinoma (HCC). We previously reported that GNMT expression is diminished in HCC. Here we report our examination of GNMT expression patterns in cholangiocarcinoma and the relationship between its expression and prognosis. METHODS: We analyzed GNMT expression in tumor tissues from 33 cholangiocarcinomapatients (19 male) using immunohistochemistry (IHC) procedures with a GNMT monoclonal antibody (mAb 4-17). GNMT expression intensity and percentages were scored on a scale of 0 to 6. The association between GNMT expression and survival was analyzed using the Kaplan-Meier method, and prognostic factors were evaluated with a multivariate Cox proportional hazards regression model. RESULTS: High GNMT expression was found in epithelial cells of normal bile ducts. Six of 33 (18.2%) cholangiocarcinoma tissues had no GNMT expression. A statistically significant difference was noted in GNMT expression between male and female patients (68.4% vs 100%, P < 0.05). Compared to patients with GNMT expression scores > 3, the death hazard ratio for patients with GNMT scores <or= 3 was 3.68 (95% confidence interval = 1.17-11.59, P < 0.05). CONCLUSIONS:GNMT expression is a favorable prognosis predictor for cholangiocarcinoma.
Authors: Amjad P Khan; Thekkelnaycke M Rajendiran; Bushra Ateeq; Irfan A Asangani; Jyoti N Athanikar; Anastasia K Yocum; Rohit Mehra; Javed Siddiqui; Ganesh Palapattu; John T Wei; George Michailidis; Arun Sreekumar; Arul M Chinnaiyan Journal: Neoplasia Date: 2013-05 Impact factor: 5.715
Authors: David Fernández-Ramos; Pablo Fernández-Tussy; Fernando Lopitz-Otsoa; Virginia Gutiérrez-de-Juan; Nicolás Navasa; Lucía Barbier-Torres; Imanol Zubiete-Franco; Jorge Simón; Agustín F Fernández; Ander Arbelaiz; Ana M Aransay; José Luis Lavín; Naiara Beraza; María J Perugorria; Jesus M Banales; Erica Villa; Mario F Fraga; Juan Anguita; Matias A Avila; Carmen Berasain; Paula Iruzibieta; Javier Crespo; Shelly C Lu; Marta Varela-Rey; José M Mato; Teresa C Delgado; María L Martínez-Chantar Journal: Cell Death Dis Date: 2018-09-20 Impact factor: 8.469