Literature DB >> 18623625

Simultaneous single-tube PCR-based assay for the direct identification of the five most common meningococcal serogroups from clinical samples.

Zacharoula Drakopoulou1, Konstantinos Kesanopoulos, Maria Sioumala, Alexandra Tambaki, Jenny Kremastinou, Georgina Tzanakaki.   

Abstract

This study presents a stepdown multiplex PCR assay for the simultaneous detection of the five most common Neisseria meningitidis serogroups (A, B, C, W-135 and Y) in 530 clinical samples obtained from 428 patients (271 blood and 259 cerebrospinal fluid). The sensitivity and the specificity was calculated to 100% [positive predictive value 100% (95%, CI 99.0-100%) and negative predictive value 100% (95% CI 99.0-100%)]. The overall effectiveness permits the rapid, accurate and inexpensive detection of the five most prevalent meningococcal serogroups in clinical samples. It is potentially a valuable tool for diagnosis and epidemiological monitoring of disease due to N. meningitidis.

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Year:  2008        PMID： 18623625     DOI： 10.1111/j.1574-695X.2008.00406.x

Source DB:  PubMed          Journal:  FEMS Immunol Med Microbiol        ISSN： 0928-8244


  7 in total

1.  Comparison of Phenotypic and Genotypic Approaches to Capsule Typing of Neisseria meningitidis by Use of Invasive and Carriage Isolate Collections.

Authors:  C Hal Jones; Naglaa Mohamed; Eduardo Rojas; Lubomira Andrew; Johanna Hoyos; Julio C Hawkins; Lisa K McNeil; Qin Jiang; Leonard W Mayer; Xin Wang; Rodica Gilca; Philippe De Wals; Louise Pedneault; Joseph Eiden; Kathrin U Jansen; Annaliesa S Anderson
Journal:  J Clin Microbiol       Date:  2015-08-26       Impact factor: 5.948

Review 2.  Epidemiology of invasive meningococcal disease in Greece, 2006-2016.

Authors:  Anastasia Flountzi; Theano Georgakopoulou; Sooria Balasegaram; Konstantinos Kesanopoulos; Athanasia Xirogianni; Anastasia Papandreou; Georgina Tzanakaki
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2019-08-15       Impact factor: 3.267

3.  Acute bacterial meningitis cases diagnosed by culture and PCR in a children's hospital throughout a 9-Year period (2000-2008) in Athens, Greece.

Authors:  Konstantina Papavasileiou; Eleni Papavasileiou; Georgina Tzanakaki; Aliki Voyatzi; Jenny Kremastinou; Stylianos Chatzipanagiotou
Journal:  Mol Diagn Ther       Date:  2011-04-01       Impact factor: 4.074

4.  Development of a multiplex PCR assay for detection and genogrouping of Neisseria meningitidis.

Authors:  Hongfei Zhu; Quan Wang; Liuqing Wen; Jianguo Xu; Zhujun Shao; Min Chen; Mingliang Chen; Peter R Reeves; Boyang Cao; Lei Wang
Journal:  J Clin Microbiol       Date:  2011-11-16       Impact factor: 5.948

5.  Meningococcal Carriage in Military Recruits and University Students during the Pre MenB Vaccination Era in Greece (2014-2015).

Authors:  Kyriaki Tryfinopoulou; Konstantinos Kesanopoulos; Athanasia Xirogianni; Nektarios Marmaras; Anastasia Papandreou; Vassiliki Papaevangelou; Maria Tsolia; Aftab Jasir; Georgina Tzanakaki
Journal:  PLoS One       Date:  2016-12-01       Impact factor: 3.240

6.  Characterization of meningococcal carriage isolates from Greece by whole genome sequencing: Implications for 4CMenB vaccine implementation.

Authors:  Konstantinos Kesanopoulos; Holly B Bratcher; Eva Hong; Athanasia Xirogianni; Anastasia Papandreou; Muhamed-Kheir Taha; Martin C J Maiden; Georgina Tzanakaki
Journal:  PLoS One       Date:  2018-12-28       Impact factor: 3.240

7.  Epidemiology of invasive meningococcal disease in Cyprus 2004 to 2018.

Authors:  Maria Koliou; Diamanto Kasapi; Stella Mazeri; Panagiota Maikanti; Anna Demetriou; Chrystalla Skordi; Maria Agathocleous; Georgina Tzanakaki; Elisavet Constantinou
Journal:  Euro Surveill       Date:  2020-07
  7 in total

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