| Literature DB >> 18621085 |
Fei Teng1, Yuntao Guan, Wanpeng Zhu.
Abstract
Fe inhibition to PCR can be overcome by adding greatly excessive EDTA first, and then the same amount Mg(2+) as EDTA to PCR mix. By this method impure DNA was amplified successfully. DNA samples containing metals whose complexation constant with EDTA was much higher than Mg(2+) can also be amplified.Entities:
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Year: 2008 PMID： 18621085 DOI： 10.1016/j.mimet.2008.06.013
Source DB: PubMed Journal: J Microbiol Methods ISSN： 0167-7012 Impact factor: 2.363