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Literature DB >> 18619499

Antibiotic marker modifications of lambda Red and FLP helper plasmids, pKD46 and pCP20, for inactivation of chromosomal genes using PCR products in multidrug-resistant strains.

Benoît Doublet¹, Gregory Douard, Hayette Targant, Danièle Meunier, Jean-Yves Madec, Axel Cloeckaert.

Abstract

The Red recombinase system of bacteriophage Lambda has been used to inactivate chromosomal genes in bacteria using PCR products. In this study, we describe the replacement of the ampicillin resistance marker of helper plasmids pKD46 and pCP20 by a gentamicin resistance gene to disrupt chromosomal genes and then to eliminate FRT flanked resistance gene in multiple antibiotic-resistant Salmonella enterica strains.

Entities: Chemical Species

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Year: 2008 PMID： 18619499 DOI： 10.1016/j.mimet.2008.06.010

Source DB: PubMed Journal: J Microbiol Methods ISSN： 0167-7012 Impact factor: 2.363

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Cited

46 in total

1. Landscape of Resistance-Nodulation-Cell Division (RND)-Type Efflux Pumps in Enterobacter cloacae Complex.

Authors: François Guérin; Claire Lallement; Christophe Isnard; Anne Dhalluin; Vincent Cattoir; Jean-Christophe Giard
Journal: Antimicrob Agents Chemother Date: 2016-03-25 Impact factor: 5.191

2. The Arginine Deiminase Operon Is Responsible for a Fitness Trade-Off in Extended-Spectrum-β-Lactamase-Producing Strains of Escherichia coli.

Authors: Typhaine Billard-Pomares; Olivier Clermont; Miguel Castellanos; Fatma Magdoud; Guilhem Royer; Bénédicte Condamine; Stéphanie Fouteau; Valérie Barbe; David Roche; Stéphane Cruveiller; Claudine Médigue; Dominique Pognard; Jeremy Glodt; Sara Dion; Odile Rigal; Bertrand Picard; Erick Denamur; Catherine Branger
Journal: Antimicrob Agents Chemother Date: 2019-07-25 Impact factor: 5.191

10. The FeoC protein leads to high cellular levels of the Fe(II) transporter FeoB by preventing FtsH protease regulation of FeoB in Salmonella enterica.

Authors: Hyunkeun Kim; Hwiseop Lee; Dongwoo Shin
Journal: J Bacteriol Date: 2013-05-24 Impact factor: 3.490

Antibiotic marker modifications of lambda Red and FLP helper plasmids, pKD46 and pCP20, for inactivation of chromosomal genes using PCR products in multidrug-resistant strains.

1. Landscape of Resistance-Nodulation-Cell Division (RND)-Type Efflux Pumps in Enterobacter cloacae Complex.

2. The Arginine Deiminase Operon Is Responsible for a Fitness Trade-Off in Extended-Spectrum-β-Lactamase-Producing Strains of Escherichia coli.

3. A method for generating precise gene deletions and insertions in Escherichia coli.

Review 4. Gene replacement techniques for Escherichia coli genome modification.

5. Red recombinase assisted gene replacement in Klebsiella pneumoniae.

6. Complex Regulation Pathways of AmpC-Mediated β-Lactam Resistance in Enterobacter cloacae Complex.

7. Ethylene glycol and glycolic acid production from xylonic acid by Enterobacter cloacae.

8. Phage Mu Gam protein promotes NHEJ in concert with Escherichia coli ligase.

9. New strategy to improve efficiency for gene replacement in Klebsiella pneumoniae.

10. The FeoC protein leads to high cellular levels of the Fe(II) transporter FeoB by preventing FtsH protease regulation of FeoB in Salmonella enterica.