[Investigation and application of fluorescence reaction of cerium-L-tryptophan in Triton X-100 microemulsion].

In the present article, the sensitizing effect of Triton X-100 microemulsion media (Triton X-100-M) on the determination of L-tryptophan by fluorescence spectrophotometry was studied, which was based on a strong fluorescence substance produced when L-tryptophan was oxidized by Ce4+ in acid medium. Compared with Triton X-100 micelle (Triton X-100-m), it was found that in the medium of Triton X-100-M the intensity of fluorescence was enhanced by 225 times. Various factors that influence fluorescence determination and interfering ions were examined. The mechanism of sensitizing effect of Triton X-100 microemulsion was also discussed. Under the optimum conditions, the liner range and detection limit are 0.1-1.0 g x mL(-1) and 0.09 microg x mL(-1) (correlation coefficient r = 0.998 4), respectively. The linear equation was F = 342.37+30.45c. The proposed method has been successfully applied to determine food samples.