BACKGROUND: Previous investigations of the protein kinase C zeta (PRKCZ) gene, a susceptibility factor for type 2 diabetes mellitus (T2DM), have focussed on its single nucleotide polymorphisms (SNPs). In a departure from these earlier studies, we tested six tagging markers of PRKCZ based on the Hapmap database to evaluate their association with T2DM. METHODS: PCR-RFLP was performed to test the genotypes in type 2 diabetes patients (n = 343) and normal controls (n = 429). Insulin-related indexes were calculated from levels of fasting plasma insulin (FPI) and fasting plasma glucose (FPG). RESULTS: The variant genotypes, rs3753242TT, rs2503706CT, rs2503706CT/ TT and rs262654TT were associated with T2DM. And the significance remained for rs3753242 TT (p < 0.001) after the false discovery rate (FDR) correction. There was a doubling in the prevalence of the TTGCCC haplotype in the case subjects (14.2%) compared with the control subjects (6.9%) [OR (95% CI) = 2.19(1.49-3.22)]. In the control group, none of the haplotypes studied was associated with insulin-related indexes. CONCLUSIONS: The PRKCZ gene variants associated with the development of T2DM in this study must be investigated in a larger population to reveal any potential effects on metabolism.
BACKGROUND: Previous investigations of the protein kinase C zeta (PRKCZ) gene, a susceptibility factor for type 2 diabetes mellitus (T2DM), have focussed on its single nucleotide polymorphisms (SNPs). In a departure from these earlier studies, we tested six tagging markers of PRKCZ based on the Hapmap database to evaluate their association with T2DM. METHODS: PCR-RFLP was performed to test the genotypes in type 2 diabetespatients (n = 343) and normal controls (n = 429). Insulin-related indexes were calculated from levels of fasting plasma insulin (FPI) and fasting plasma glucose (FPG). RESULTS: The variant genotypes, rs3753242TT, rs2503706CT, rs2503706CT/ TT and rs262654TT were associated with T2DM. And the significance remained for rs3753242 TT (p < 0.001) after the false discovery rate (FDR) correction. There was a doubling in the prevalence of the TTGCCC haplotype in the case subjects (14.2%) compared with the control subjects (6.9%) [OR (95% CI) = 2.19(1.49-3.22)]. In the control group, none of the haplotypes studied was associated with insulin-related indexes. CONCLUSIONS: The PRKCZ gene variants associated with the development of T2DM in this study must be investigated in a larger population to reveal any potential effects on metabolism.
Authors: Jayalakshmi Lakshmipathi; Juan Carlos Alvarez-Perez; Carolina Rosselot; Gabriella P Casinelli; Rachel E Stamateris; Francisco Rausell-Palamos; Christopher P O'Donnell; Rupangi C Vasavada; Donald K Scott; Laura C Alonso; Adolfo Garcia-Ocaña Journal: Diabetes Date: 2016-02-11 Impact factor: 9.461