Literature DB >> 18615522

Stabilizing effect of amphiphilic excipients on the freeze-thawing and freeze-drying of lactate dehydrogenase.

K Izutsu1, S Yoshioka, T Terao.   

Abstract

The effects of amphiphilic excipients on the inactivation of lactate dehydrogenase (LDH) during freeze-thawing and freeze-drying were studied. Some amphiphilic excipients such as hydroxypropyl-beta-cyclodextrin (HP-beta-CD), CHAPS, polyethylene glycol (PEG) 3350, and sucrose fatty acid monoester prevented LDH inactivation during freeze-thawing and freeze-drying at a lower concentration than sugars and amino acids. Polyoxyethylene 9 lauryl ether and PEG 400 protected LDH during freeze-thawing but not during freeze-drying. The buffer concentration of the solution to be freeze-dried (10, 50, and 200 mM) affected the stabilizing effect of trehalose, but not that of HP-beta-CD. (c) 1994 John Wiley & Sons, Inc.

Entities:  

Year:  1994        PMID： 18615522     DOI： 10.1002/bit.260431114

Source DB:  PubMed          Journal:  Biotechnol Bioeng        ISSN： 0006-3592            Impact factor:   4.530


  7 in total

1.  Stabilization of lactate dehydrogenase following freeze thawing and vacuum-drying in the presence of trehalose and borate.

Authors:  D P Miller; R E Anderson; J J de Pablo
Journal:  Pharm Res       Date:  1998-08       Impact factor: 4.200

2.  Protecting activity of desiccated enzymes.

Authors:  Samantha Piszkiewicz; Kathryn H Gunn; Owen Warmuth; Ashlee Propst; Aakash Mehta; Kenny H Nguyen; Elizabeth Kuhlman; Alex J Guseman; Samantha S Stadmiller; Thomas C Boothby; Saskia B Neher; Gary J Pielak
Journal:  Protein Sci       Date:  2019-03-30       Impact factor: 6.725

Review 3.  Protecting Enzymes from Stress-Induced Inactivation.

Authors:  Samantha Piszkiewicz; Gary J Pielak
Journal:  Biochemistry       Date:  2019-09-05       Impact factor: 3.162

4.  Preparation, Characterization, and Mechanism of Antifreeze Peptides from Defatted Antarctic Krill (Euphausia superba) on Lactobacillus rhamnosus.

Authors:  Yu Liu; Xuena Yu; Yanling Zhu; Wei Yang; Yan Zeng; Yi Hu; Wei Jiang
Journal:  Molecules       Date:  2022-04-26       Impact factor: 4.927

5.  Increased stabilizing effects of amphiphilic excipients on freeze-drying of lactate dehydrogenase (LDH) by dispersion into sugar matrices.

Authors:  K Izutsu; S Yoshioka; S Kojima
Journal:  Pharm Res       Date:  1995-06       Impact factor: 4.200

6.  A beetle antifreeze protein protects lactate dehydrogenase under freeze-thawing.

Authors:  Celeste Rodriguez; Seyed Sajjadi; Ravinder Abrol; Xin Wen
Journal:  Int J Biol Macromol       Date:  2019-06-19       Impact factor: 8.025

7.  Protection of Alcohol Dehydrogenase against Freeze-Thaw Stress by Ice-Binding Proteins Is Proportional to Their Ice Recrystallization Inhibition Property.

Authors:  Young Hoon Lee; Kitae Kim; Jun Hyuck Lee; Hak Jun Kim
Journal:  Mar Drugs       Date:  2020-12-13       Impact factor: 5.118
  7 in total

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