Literature DB >> 1861458

Confocal scanning laser microscopy of mitochondria: a possible tool in the diagnosis of mitochondrial disorders.

M H Ruiters1, E A van Spronsen, O H Skjeldal, P Stromme, H R Scholte, H Pzyrembel, G P Smit, W Ruitenbeek, E Agsteribbe.   

Abstract

This paper describes a non-invasive method for the study of mitochondrial morphology in cultured human skin fibroblasts by confocal scanning laser microscopy after staining the mitochondria with 2-[4-(dimethylaminostyryl]-1-methylpyridinium iodide. This method is applied to compare mitochondria in fibroblasts from healthy individuals and from patients with mitochondrial myopathy. In most cases there is a striking swelling of the patient's mitochondria and a loss of fine structure. The results with respect to the potential of this method as a diagnostic tool are discussed.

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Year:  1991        PMID： 1861458     DOI： 10.1007/bf01804387

Source DB:  PubMed          Journal:  J Inherit Metab Dis        ISSN： 0141-8955            Impact factor:   4.982


  5 in total

1.  Fluorimetry of mitochondria in cells vitally stained with DASPMI or rhodamine 6 GO.

Authors:  J Bereiter-Hahn; K H Seipel; M Vöth; J S Ploem
Journal:  Cell Biochem Funct       Date:  1983-10       Impact factor: 3.685

2.  Three-dimensional chromatin distribution in neuroblastoma nuclei shown by confocal scanning laser microscopy.

Authors:  G J Brakenhoff; H T van der Voort; E A van Spronsen; W A Linnemans; N Nanninga
Journal:  Nature       Date:  1985 Oct 24-30       Impact factor: 49.962

Review 3.  Morphological observations in skeletal muscle from patients with a mitochondrial myopathy.

Authors:  A M Stadhouders; R C Sengers
Journal:  J Inherit Metab Dis       Date:  1987       Impact factor: 4.982

4.  Studies on the formation of lactate and pyruvate from glucose in cultured skin fibroblasts: implications for detection of respiratory chain defects.

Authors:  F A Wijburg; N Feller; H R Scholte; H Przyrembel; R J Wanders
Journal:  Biochem Int       Date:  1989-09

5.  Dimethylaminostyrylmethylpyridiniumiodine (daspmi) as a fluorescent probe for mitochondria in situ.

Authors:  J Bereiter-Hahn
Journal:  Biochim Biophys Acta       Date:  1976-01-15
  5 in total
  2 in total

1.  A new case of multiple mitochondrial enzyme deficiencies with decreased amount of heat shock protein 60.

Authors:  P Briones; M A Vilaseca; A Ribes; A Vernet; M Lluch; V Cusi; A Huckriede; E Agsteribbe
Journal:  J Inherit Metab Dis       Date:  1997-08       Impact factor: 4.982

2.  Morphology of the mitochondria in heat shock protein 60 deficient fibroblasts from mitochondrial myopathy patients. Effects of stress conditions.

Authors:  A Huckriede; A Heikema; K Sjollema; P Briones; E Agsteribbe
Journal:  Virchows Arch       Date:  1995       Impact factor: 4.064
  2 in total

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