| Literature DB >> 18614088 |
L Van Vloten-Doting1, E M Jaspars.
Abstract
RNA was extracted from alfalfa mosaic virus 425. Infectivity was determined in density gradient fractions and in all possible combinations of two fractions. Certain combinations were remarkably infectious. This led to the purification through two cycles of sucrose gradient centrifugation of a 27 S and a 14 S RNA, from bottom component and top component a, respectively. The purified bottom component RNA had a low infectivity; when purified top component a RNA was added, infectivity increased considerably. Possible explanations for this effect are discussed.Entities:
Year: 1967 PMID： 18614088 DOI： 10.1016/0042-6822(67)90068-2
Source DB: PubMed Journal: Virology ISSN： 0042-6822 Impact factor: 3.616