S O Mashayekhi1, M Ghandforoush-Sattari, R D W Hain. 1. Faculty of Pharmacy and Drug Applied Research Center, Hematology & Oncology Research Centre, NPMC, Tabriz University of Medical Sciences, Tabriz, Iran. mashayekhis@yahoo.co.uk
Abstract
BACKGROUND: Morphine is one of the most widely used opioid analgesics for controlling pain in cancer and post-operative patients. OBJECTIVE: This study aimed at finding a sensitive method for measuring morphine. METHOD: A one-step solid phase extraction was developed for extracting morphine from various samples, and morphine concentration was measured using a high-performance liquid chromatographic system with electrochemical detection. The sensitivity of the assay was 1.53 ng/mL with a recovery of 93.4% +/- 0.01. The mean intra-assay and inter-assay test for three concentrations was 10.54 and 7.47, respectively. The assay showed no cross-reaction with a wide range of compounds. CONCLUSION: This method for morphine in small biological samples is easy, sensitive and reproducible with low cross-reactivity.
BACKGROUND:Morphine is one of the most widely used opioid analgesics for controlling pain in cancer and post-operative patients. OBJECTIVE: This study aimed at finding a sensitive method for measuring morphine. METHOD: A one-step solid phase extraction was developed for extracting morphine from various samples, and morphine concentration was measured using a high-performance liquid chromatographic system with electrochemical detection. The sensitivity of the assay was 1.53 ng/mL with a recovery of 93.4% +/- 0.01. The mean intra-assay and inter-assay test for three concentrations was 10.54 and 7.47, respectively. The assay showed no cross-reaction with a wide range of compounds. CONCLUSION: This method for morphine in small biological samples is easy, sensitive and reproducible with low cross-reactivity.