Literature DB >> 18609088

Difficulties for primary health care staff in interpreting bacterial findings on a device for simplified urinary culture.

Elisabeth Palmqvist1, Olle Aspevall, Eva Burman, Gunnar Nordin, Anita Svahn, Urban Forsum.   

Abstract

The reliability of interpretations of findings from dip-slide devices for culturing urine was investigated in a national Swedish external quality assessment (EQA) programme. Also investigated was the extent of improvement in the examination procedure achieved through personnel training programmes and information. According to Swedish national recommendations, dip-slide should only be used in primary health care (PHC) in cases of uncomplicated urinary tract infection (UTI) in females of childbearing age. The recommendations also define six possible outcomes of a dip-slide examination, outcomes that have formed the basis for the EQA programme since 2001. No improvement in ability to classify readings correctly into the six categories was noted for the period 2001 to 2006. Preparations containing 'mixed flora' presented participants with the greatest difficulty, with only 28 % correct reports. The EQA programme, with educational components and voluntary participation, has not improved quality. The disappointing results might be a reflection of the limited effort and resources allocated by clinical microbiology laboratories for training and for sustaining proficiency in the evaluation of dip-slides. For these reasons, we cannot at present recommend the dip-slide technique for use in PHC settings.

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Year:  2008        PMID: 18609088     DOI: 10.1080/00365510701759703

Source DB:  PubMed          Journal:  Scand J Clin Lab Invest        ISSN: 0036-5513            Impact factor:   1.713


  6 in total

1.  Analytic laboratory performance of a point of care urine culture kit for diagnosis and antibiotic susceptibility testing.

Authors:  E Bongard; N Frimodt-Møller; M Gal; M Wootton; R Howe; N Francis; H Goossens; C C Butler
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2015-08-06       Impact factor: 3.267

2.  Rapid and sensitive detection of major uropathogens in a single-pot multiplex PCR assay.

Authors:  B Padmavathy; R Vinoth Kumar; Amee Patel; S Deepika Swarnam; T Vaidehi; B M Jaffar Ali
Journal:  Curr Microbiol       Date:  2012-04-22       Impact factor: 2.188

3.  Rapid qualitative urinary tract infection pathogen identification by SeptiFast real-time PCR.

Authors:  Lutz E Lehmann; Stefan Hauser; Thomas Malinka; Sven Klaschik; Stefan U Weber; Jens-Christian Schewe; Frank Stüber; Malte Book
Journal:  PLoS One       Date:  2011-02-16       Impact factor: 3.240

4.  Rapid discrimination of Gram-positive and Gram-negative bacteria in liquid samples by using NaOH-sodium dodecyl sulfate solution and flow cytometry.

Authors:  Atsushi Wada; Mari Kono; Sawako Kawauchi; Yuri Takagi; Takashi Morikawa; Kunihiro Funakoshi
Journal:  PLoS One       Date:  2012-10-15       Impact factor: 3.240

5.  Optimization and evaluation of Flexicult® Vet for detection, identification and antimicrobial susceptibility testing of bacterial uropathogens in small animal veterinary practice.

Authors:  Luca Guardabassi; Sandra Hedberg; Lisbeth Rem Jessen; Peter Damborg
Journal:  Acta Vet Scand       Date:  2015-10-26       Impact factor: 1.695

Review 6.  A New Gold Rush: A Review of Current and Developing Diagnostic Tools for Urinary Tract Infections.

Authors:  Raymond Xu; Nicholas Deebel; Randy Casals; Rahul Dutta; Majid Mirzazadeh
Journal:  Diagnostics (Basel)       Date:  2021-03-09
  6 in total

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