PURPOSE: To elucidate the thermal impact on biological processes the first three hours after hyperthermia (HT). MATERIALS AND METHODS: Tumor samples from BT(4)An rat tumors were transplanted subcutaneously to the right hind foot of BD IX rats, treated with local water-bath HT (mean tumor temperature of 43 degrees C) for one hour, and analyzed for changes in global gene expression the first three hours after treatment with HT. Samples from a corresponding in vitro experiment were also analyzed. Differentially expressed genes in vivo were mapped to a gene ontology (GO) database in search for biological processes overrepresented after treatment with HT. Selected genes were verified using Taqman quantitative RT-PCR. RESULTS: 1213 genes were differentially expressed after HT treatment in vivo. Processes overrepresented by GO mapping could be sorted into 10 main functional groups; apoptosis, transcription, immune system, blood vessels, metabolism/protein modifications, differentiation, cell signaling, response to stimulus, transport and cytoskeleton. We detected reduced mRNA levels of a wide range of T-cell, natural killer cell and antigen presenting cell-related genes after HT. CONCLUSION: To our best knowledge, this is the first global microarray analysis of a malignant tumor treated with HT in vivo. We highlight biological processes affected by HT. RNA expression profiles also indicate a rapid suppression of the immune system within tumors by local HT.
PURPOSE: To elucidate the thermal impact on biological processes the first three hours after hyperthermia (HT). MATERIALS AND METHODS:Tumor samples from BT(4)An rattumors were transplanted subcutaneously to the right hind foot of BD IX rats, treated with local water-bath HT (mean tumor temperature of 43 degrees C) for one hour, and analyzed for changes in global gene expression the first three hours after treatment with HT. Samples from a corresponding in vitro experiment were also analyzed. Differentially expressed genes in vivo were mapped to a gene ontology (GO) database in search for biological processes overrepresented after treatment with HT. Selected genes were verified using Taqman quantitative RT-PCR. RESULTS: 1213 genes were differentially expressed after HT treatment in vivo. Processes overrepresented by GO mapping could be sorted into 10 main functional groups; apoptosis, transcription, immune system, blood vessels, metabolism/protein modifications, differentiation, cell signaling, response to stimulus, transport and cytoskeleton. We detected reduced mRNA levels of a wide range of T-cell, natural killer cell and antigen presenting cell-related genes after HT. CONCLUSION: To our best knowledge, this is the first global microarray analysis of a malignant tumor treated with HT in vivo. We highlight biological processes affected by HT. RNA expression profiles also indicate a rapid suppression of the immune system within tumors by local HT.
Authors: Jen-Tsan Chi; Donald E Thrall; Chen Jiang; Stacey Snyder; Diane Fels; Chelsea Landon; Linda McCall; Lan Lan; Marlene Hauck; James R MacFall; Benjamin L Viglianti; Mark W Dewhirst Journal: Clin Cancer Res Date: 2011-02-03 Impact factor: 12.531
Authors: Caecilia Wismeth; Christine Dudel; Christina Pascher; Paul Ramm; Torsten Pietsch; Birgit Hirschmann; Christiane Reinert; Martin Proescholdt; Petra Rümmele; Gerhard Schuierer; Ulrich Bogdahn; Peter Hau Journal: J Neurooncol Date: 2009-12-24 Impact factor: 4.130