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Literature DB >> 1860831

Transcriptional analysis of the Staphylococcus aureus plasmid pI258 mercury resistance determinant.

Abstract

Northern blot DNA-RNA hybridization analysis of Staphylococcus aureus cells bearing pI258 showed that upon induction the amount of mer operon transcript present increased 49-fold over that observed in uninduced cells. Maximum induction occurred after 45 min in the presence of 5 microM HgCl2. Two transcripts, 5.0 and 5.8 kb long, were observed. Both transcripts encoded merR through merB (inclusive). Primer extension analysis determined that the 5' end of at least one transcript (and presumably of both) started at a T or G, 7 or 8 nucleotides downstream from the putative -10 site.

Entities: Chemical Species

Mesh：

Substances：
Mercury

Year: 1991 PMID： 1860831 PMCID： PMC208219 DOI： 10.1128/jb.173.16.5234-5238.1991

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

17 in total

1. The phoBR operon in Escherichia coli K-12.

Authors: B L Wanner; B D Chang
Journal: J Bacteriol Date: 1987-12 Impact factor: 3.490

2. Nucleotide sequence of ermA, a macrolide-lincosamide-streptogramin B determinant in Staphylococcus aureus.

Authors: E Murphy
Journal: J Bacteriol Date: 1985-05 Impact factor: 3.490

3. A rapid method to quantitate non-labeled RNA species in bacterial cells.

Authors: J S Kornblum; S J Projan; S L Moghazeh; R P Novick
Journal: Gene Date: 1988 Impact factor: 3.688

4. Transcriptional switching by the metalloregulatory MerR protein: initial characterization of DNA and mercury (II) binding activities.

Authors: L M Shewchuk; G L Verdine; C T Walsh
Journal: Biochemistry Date: 1989-03-07 Impact factor: 3.162

5. Mercury and organomercurial resistances determined by plasmids in Staphylococcus aureus.

Authors: A A Weiss; S D Murphy; S Silver
Journal: J Bacteriol Date: 1977-10 Impact factor: 3.490

6. Nucleotide sequence and expression of the mercurial-resistance operon from Staphylococcus aureus plasmid pI258.

Authors: R A Laddaga; L Chu; T K Misra; S Silver
Journal: Proc Natl Acad Sci U S A Date: 1987-08 Impact factor: 11.205

7. The gluconate operon gnt of Bacillus subtilis encodes its own transcriptional negative regulator.

Authors: Y Fujita; T Fujita
Journal: Proc Natl Acad Sci U S A Date: 1987-07 Impact factor: 11.205

8. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.

Authors: C Yanisch-Perron; J Vieira; J Messing
Journal: Gene Date: 1985 Impact factor: 3.688

9. Molecular basis for resistance to antimycin and diuron, Q-cycle inhibitors acting at the Qi site in the mitochondrial ubiquinol-cytochrome c reductase in Saccharomyces cerevisiae.

Authors: J P di Rago; A M Colson
Journal: J Biol Chem Date: 1988-09-05 Impact factor: 5.157

Transcriptional analysis of the Staphylococcus aureus plasmid pI258 mercury resistance determinant.

1. The phoBR operon in Escherichia coli K-12.

2. Nucleotide sequence of ermA, a macrolide-lincosamide-streptogramin B determinant in Staphylococcus aureus.

3. A rapid method to quantitate non-labeled RNA species in bacterial cells.

4. Transcriptional switching by the metalloregulatory MerR protein: initial characterization of DNA and mercury (II) binding activities.

5. Mercury and organomercurial resistances determined by plasmids in Staphylococcus aureus.

6. Nucleotide sequence and expression of the mercurial-resistance operon from Staphylococcus aureus plasmid pI258.

7. The gluconate operon gnt of Bacillus subtilis encodes its own transcriptional negative regulator.

8. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.

9. Molecular basis for resistance to antimycin and diuron, Q-cycle inhibitors acting at the Qi site in the mitochondrial ubiquinol-cytochrome c reductase in Saccharomyces cerevisiae.

10. Cloning, characterization, and sequencing of an accessory gene regulator (agr) in Staphylococcus aureus.

1. Regulation of the Staphylococcus aureus plasmid pI258 mercury resistance operon.

Review 2. Untwist and shout: a heavy metal-responsive transcriptional regulator.

Review 3. Gene regulation of plasmid- and chromosome-determined inorganic ion transport in bacteria.

4. Mercury resistance in Bacillus cereus RC607: transcriptional organization and two new open reading frames.

5. Regulation of Rot expression in Staphylococcus aureus.