Literature DB >> 186030

Myosin light-chain phosphatase.

M Morgan, S V Perry, J Ottaway.   

Abstract

1. A method for the isolation of a new enzyme, myosin light-chain phosphatase, from rabbit white skeletal muscle by using a Sepharose-phosphorylated myosin light-chain affinity column is described. 2. The enzyme migrated as a single component on electrophoresis in sodium dodecyl sulphate/polyacrylamide gel at pH7.0, with apparent mol.wt. 70000. 3. The enzyme was highly specific for the phosphorylated P-light chain of myosin, had pH optima at 6.5 and 8.0 and was not inhibited by NaF. 4. A Ca2+-sensitive 'ATPase' (adenosine triphosphatase) system consisting of myosin light-chain kinase, myosin light-chain phosphatase and the P-light chain is described. 5. Evidence is presented for a phosphoryl exchange between Pi, phosphorylated P-light chain and myosin light-chain phosphatase. 6. Heavy meromyosin prepared by chymotryptic digestion can be phosphorylated by myosin light-chain kinase. 7. The ATPase activities of myosin and heavy meromyosin, in the presence and absence of F-actin, were not significantly changed (+/- 10%) by phosphorylation of the P-light chain.

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Year:  1976        PMID： 186030      PMCID： PMC1163911          DOI： 10.1042/bj1570687

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  29 in total

1.  Insulin treatment and increased UDPG-glycogen transglucosylase activity in muscle.

Authors:  C VILLAR-PALASI; J LARNER
Journal:  Arch Biochem Biophys       Date:  1961-09       Impact factor: 4.013

2.  The subunits and biological activity of polymorphic forms of tropomyosin.

Authors:  P Cummins; S V Perry
Journal:  Biochem J       Date:  1973-08       Impact factor: 3.857

3.  Phosphorylation of the "37000 component" of the troponin complex (troponin-t).

Authors:  S V Perry; H A Cole
Journal:  Biochem J       Date:  1973-02       Impact factor: 3.857

4.  A simplified method for cyanogen bromide activation of agarose for affinity chromatography.

Authors:  S C March; I Parikh; P Cuatrecasas
Journal:  Anal Biochem       Date:  1974-07       Impact factor: 3.365

5.  Myosin light-chain kinase, a new enzyme from striated muscle.

Authors:  E Pires; S V Perry; M A Thomas
Journal:  FEBS Lett       Date:  1974-05-01       Impact factor: 4.124

6.  A phosphorylated light-chain component of myosin from skeletal muscle.

Authors:  W T Perrie; L B Smillie; S B Perry
Journal:  Biochem J       Date:  1973-09       Impact factor: 3.857

7.  Troponin and its components.

Authors:  S Ebashi; T Wakabayashi; F Ebashi
Journal:  J Biochem       Date:  1971-02       Impact factor: 3.387

8.  The gel-filtration behaviour of proteins related to their molecular weights over a wide range.

Authors:  P Andrews
Journal:  Biochem J       Date:  1965-09       Impact factor: 3.857

9.  The chromatography of L-myosin on diethylaminoethylcellulose.

Authors:  S V PERRY
Journal:  Biochem J       Date:  1960-01       Impact factor: 3.857

10.  3-methylhistidine in actin and other muscle proteins.

Authors:  P Johnson; C I Harris; S V Perry
Journal:  Biochem J       Date:  1967-10       Impact factor: 3.857
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  35 in total

1.  A hypothalamic activator of calmodulin-dependent enzymes is thymosin beta 4 (1-39).

Authors:  A A Galoyan; L A Shuvalova; M T Davis; J E Shively; T D Lee
Journal:  Neurochem Res       Date:  1992-08       Impact factor: 3.996

2.  X-ray diffraction study of the structural changes accompanying phosphorylation of tarantula muscle.

Authors:  R Padrón; N Panté; H Sosa; J Kendrick-Jones
Journal:  J Muscle Res Cell Motil       Date:  1991-06       Impact factor: 2.698

3.  Myosin regulatory light chain phosphorylation inhibits shortening velocities of skeletal muscle fibers in the presence of the myosin inhibitor blebbistatin.

Authors:  Melanie Stewart; Kathy Franks-Skiba; Roger Cooke
Journal:  J Muscle Res Cell Motil       Date:  2009-01-06       Impact factor: 2.698

4.  Effect of phosphorylation of smooth muscle myosin on actin activation and Ca2+ regulation.

Authors:  S Chacko; M A Conti; R S Adelstein
Journal:  Proc Natl Acad Sci U S A       Date:  1977-01       Impact factor: 11.205

5.  Non-correlation of phosphorylation of the P-light chain and the actin activation of the ATPase of chicken gizzard myosin.

Authors:  H A Cole; R J Grand; S V Perry
Journal:  Biochem J       Date:  1982-08-15       Impact factor: 3.857

6.  Inhibitory effect of a marine-sponge toxin, okadaic acid, on protein phosphatases. Specificity and kinetics.

Authors:  C Bialojan; A Takai
Journal:  Biochem J       Date:  1988-11-15       Impact factor: 3.857

7.  Greatwall-phosphorylated Endosulfine is both an inhibitor and a substrate of PP2A-B55 heterotrimers.

Authors:  Byron C Williams; Joshua J Filter; Kristina A Blake-Hodek; Brian E Wadzinski; Nicholas J Fuda; David Shalloway; Michael L Goldberg
Journal:  Elife       Date:  2014-03-11       Impact factor: 8.140

8.  Myosin light chain kinase from skeletal muscle regulates an ATP-dependent interaction between actin and myosin by binding to actin.

Authors:  K Fujita; L H Ye; M Sato; T Okagaki; Y Nagamachi; K Kohama
Journal:  Mol Cell Biochem       Date:  1999-01       Impact factor: 3.396

9.  Phosphorylation in vivo of the P light chain of myosin in rabbit fast and slow skeletal muscles.

Authors:  S A Westwood; O Hudlicka; S V Perry
Journal:  Biochem J       Date:  1984-03-15       Impact factor: 3.857

10.  Purification and properties of myosin light-chain kinase from fast skeletal muscle.

Authors:  E M Pires; S V Perry
Journal:  Biochem J       Date:  1977-10-01       Impact factor: 3.857
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