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Literature DB >> 186029

Phosphorylation of synaptic-membrane proteins from ox cerebral cortex in vitro. Partition of substrates and protein kinase activities with triton X-100.

Abstract

Synaptic-membrane fragments from ox cerebral cortex contain basal and cyclic AMP-stimulated protein kinase activity catalysing the phosphorylation of endogenous substrates. Extraction of membrane fragments with Triton X-100 solubilized less than 20% of the kinase activity and left the major part of the endogenous substrates in the insoluble fraction.

Entities: Chemical

Mesh：

Substances：

Year: 1976 PMID： 186029 PMCID： PMC1163908 DOI： 10.1042/bj1570661

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

17 in total

1. Adenosine 3':5'-monophosphate-regulated phosphorylation of erythrocyte membrane proteins. Separation of membrane-associated cyclic adenosine 3':5'-monophosphate-dependent protein kinase from its endogenous substrates.

Authors: C S Rubin
Journal: J Biol Chem Date: 1975-12-10 Impact factor: 5.157

2. Localization in the synaptic junction of the cyclic AMP stimulated intrinsic protein kinase activity of synaptosomal plasma membranes.

Authors: M Weller; I G Morgan
Journal: Biochim Biophys Acta Date: 1976-04-16

3. Widespread occurrence of a specific protein in vertebrate tissues and regulation by cyclic AMP of its endogenous phosphorylation and dephosphorylation.

Authors: A M Malkinson; B K Krueger; S A Rudolph; J E Casnellie; B E Haley; P Greengard
Journal: Metabolism Date: 1975-03 Impact factor: 8.694

4. Regulation of endogenous phosphorylation of specific proteins in synaptic membrane fractions from rat brain by adenosine 3':5'-monophosphate.

Authors: T Ueda; H Maeno; P Greengard
Journal: J Biol Chem Date: 1973-12-10 Impact factor: 5.157

5. Phosphoprotein phosphatases from rat cerebral cortex. Subcellular distribution and characterization.

Authors: H Maeno; P Greengard
Journal: J Biol Chem Date: 1972-05-25 Impact factor: 5.157

6. Phosphorylation of endogenous protein of rat brain by cyclic adenosine 3',5'-monophosphate-dependent protein kinase.

Authors: E M Johnson; H Maeno; P Greengard
Journal: J Biol Chem Date: 1971-12-25 Impact factor: 5.157

7. Isolation of synaptic junctional complexes from rat brain.

Authors: G A Davis; F E Bloom
Journal: Brain Res Date: 1973-11-09 Impact factor: 3.252

8. An ultrastructural and chemical analysis of the effect of triton X-100 on synaptic plasma membranes.

Authors: C W Cotman; G Banker; W Levy; D Taylor
Journal: Biochim Biophys Acta Date: 1971-12-03

9. Large scale preparation of a crude membrane fraction from ox brain.

Authors: R Rodnight; M Weller; P S Goldfarb
Journal: J Neurochem Date: 1969-12 Impact factor: 5.372

10. Protein kinase activity in membrane preparations from ox brain. Stimulation of intrinsic activity by adenosine 3':5'-cyclic monophosphate.

Authors: M Weller; R Rodnight
Journal: Biochem J Date: 1973-03 Impact factor: 3.857

5 in total

1. Calcium ion-stimulated phosphorylation of myelin proteins.

Authors: P V Sulakhe; E H Petrali; B J Thiessen; E R Davis
Journal: Biochem J Date: 1980-02-15 Impact factor: 3.857

2. Phosphorylation of synaptic-membrane proteins from ox cerebral cortex in vitro. Preparation of fractions enriched in phosphorylated proteins by using extraction with detergents and urea, and gel filtration.

Authors: P R Dunkley; H Holmes; R Rodnight
Journal: Biochem J Date: 1977-05-01 Impact factor: 3.857