| Literature DB >> 18602265 |
Yoichi Takakusagi1, Yuki Kuroiwa, Fumio Sugawara, Kengo Sakaguchi.
Abstract
An 11-mer unique peptide sequence SIFPLCNSGAL was identified as a methotrexate (MTX)-binding peptide from a T7 phage display screen using a quartz-crystal microbalance (QCM) biosensor. The synthetic peptide displayed weak interaction with MTX (K(D) 2.23x10(-5) M) using surface plasmon resonance (SPR). Interestingly, analysis of the primary amino acid sequence of the peptide identified similarities to the MTX-binding site of dihydrofolate reductase (DHFR). Our results highlight the importance of this primary sequence for the recognition of the MTX molecule.Entities:
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Year: 2008 PMID： 18602265 DOI： 10.1016/j.bmc.2008.06.007
Source DB: PubMed Journal: Bioorg Med Chem ISSN： 0968-0896 Impact factor: 3.641