| Literature DB >> 18601537 |
Wataru Watanabe1, Sachihiro Matsunaga, Tsunehito Higashi, Kiichi Fukui, Kazuyoshi Itoh.
Abstract
Femtosecond laser pulses in the near-infrared region have potential applications in the imaging and manipulation of intracellular organelles. We report on the manipulation of intracellular organelles by two-photon excitation. The dynamics of green fluorescent protein (GFP)-histone are investigated by two-photon fluorescence recovery after photobleaching (FRAP). Intracellular ablation of fluorescently labeled organelles in living cells is performed by focusing femtosecond laser pulses. We report on the selective marking of individual organelles by using two-photon conversion of a photoconvertible fluorescent protein.Mesh：
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Year: 2008 PMID： 18601537 DOI： 10.1117/1.2939401
Source DB: PubMed Journal: J Biomed Opt ISSN： 1083-3668 Impact factor: 3.170