PURPOSE: To compare instruments and reagents used for cytometric bead-based assay (CBA) of cytokines in human tears. METHODS: Two CBA instruments and reagent kits from five vendors were compared in parallel. Vendor protocols were modified to accommodate tear volumes. Precision, recovery, dilution linearity, and stability were evaluated. RESULTS: Luminextrade mark and Bio-Radtrade mark proved optimal for tear CBA. Good correlation for dilution linearity was observed (r > 0.80, p < 0.05) for 1-4-mu l sample volumes. Samples were stable for 30 min at 25 degrees C. CONCLUSION: Many tear cytokines can be reliably measured by CBA using an optimized protocol. Concentrations remain stable during periods consistent with collection.
PURPOSE: To compare instruments and reagents used for cytometric bead-based assay (CBA) of cytokines in human tears. METHODS: Two CBA instruments and reagent kits from five vendors were compared in parallel. Vendor protocols were modified to accommodate tear volumes. Precision, recovery, dilution linearity, and stability were evaluated. RESULTS: Luminextrade mark and Bio-Radtrade mark proved optimal for tear CBA. Good correlation for dilution linearity was observed (r > 0.80, p < 0.05) for 1-4-mu l sample volumes. Samples were stable for 30 min at 25 degrees C. CONCLUSION: Many tear cytokines can be reliably measured by CBA using an optimized protocol. Concentrations remain stable during periods consistent with collection.
Authors: Mark D P Willcox; Pablo Argüeso; Georgi A Georgiev; Juha M Holopainen; Gordon W Laurie; Tom J Millar; Eric B Papas; Jannick P Rolland; Tannin A Schmidt; Ulrike Stahl; Tatiana Suarez; Lakshman N Subbaraman; Omür Ö Uçakhan; Lyndon Jones Journal: Ocul Surf Date: 2017-07-20 Impact factor: 5.033
Authors: Meera F Iyengar; Luis F Soto; David Requena; Andrea O Ruiz-Alejos; Yvonne Huaylinos; Ricardo Velasquez; Antonio Bernabe-Ortiz; Robert H Gilman Journal: Diabetes Res Clin Pract Date: 2020-04-10 Impact factor: 5.602