OBJECTIVE AND DESIGN: In the presented study we compared the effect of stable peptide BPC 157 and methylprednisolone on early functional recovery after Achilles tendon to bone transection in a rat model before collagen healing started. MATERIAL AND METHODS: Surgical transection of the right Achilles tendon to bone area was performed in seventy two Wistar Albino male rats. Healing Achilles tendon edges were harvested at days 1-4 following the transection. Using Achilles functional index (AFI), myeloperoxidase activity, histological inflammatory cell influx and vascular index early functional recovery was evaluated. TREATMENT: Agents (stable peptide BPC 157 10 microg methylprednisolone 5 mg, normal saline 5 ml) were given alone (/kg b.w., intraperitoneally, once daily, first 30 min after surgery, last 24 h before analysis). Control group received normal saline 5 ml/kg. RESULTS: BPC 157 improved functional recovery (AFI values increased at all time points, p <0.05) by anti-inflammatory (decreased myeloperoxidase (MPO) activity and histological inflammatory cell influx, p <0.05) and increased new blood vessel formation (increased vascular index, p <0.05). Methyprednisolone decreased MPO activity and histological inflammatory cell influx, (p <0.05) but also decreased new blood vessel formation and did not affect early functional recovery. CONCLUSIONS: Stable peptide BPC 157 with combined anti-inflammatory action and induction of early new blood vessel formation facilitates early functional recovery in Achilles tendon to bone healing.
OBJECTIVE AND DESIGN: In the presented study we compared the effect of stable peptide BPC 157 and methylprednisolone on early functional recovery after Achilles tendon to bone transection in a rat model before collagen healing started. MATERIAL AND METHODS: Surgical transection of the right Achilles tendon to bone area was performed in seventy two Wistar Albino male rats. Healing Achilles tendon edges were harvested at days 1-4 following the transection. Using Achilles functional index (AFI), myeloperoxidase activity, histological inflammatory cell influx and vascular index early functional recovery was evaluated. TREATMENT: Agents (stable peptide BPC 157 10 microg methylprednisolone 5 mg, normal saline 5 ml) were given alone (/kg b.w., intraperitoneally, once daily, first 30 min after surgery, last 24 h before analysis). Control group received normal saline 5 ml/kg. RESULTS:BPC 157 improved functional recovery (AFI values increased at all time points, p <0.05) by anti-inflammatory (decreased myeloperoxidase (MPO) activity and histological inflammatory cell influx, p <0.05) and increased new blood vessel formation (increased vascular index, p <0.05). Methyprednisolone decreased MPO activity and histological inflammatory cell influx, (p <0.05) but also decreased new blood vessel formation and did not affect early functional recovery. CONCLUSIONS: Stable peptide BPC 157 with combined anti-inflammatory action and induction of early new blood vessel formation facilitates early functional recovery in Achilles tendon to bone healing.
Authors: Predrag Sikiric; Ki-Baik Hahm; Alenka Boban Blagaic; Ante Tvrdeic; Katarina Horvat Pavlov; Andrea Petrovic; Antonio Kokot; Slaven Gojkovic; Ivan Krezic; Domagoj Drmic; Rudolf Rucman; Sven Seiwerth Journal: Gut Liver Date: 2020-03-15 Impact factor: 4.519
Authors: Sven Seiwerth; Marija Milavic; Jaksa Vukojevic; Slaven Gojkovic; Ivan Krezic; Lovorka Batelja Vuletic; Katarina Horvat Pavlov; Andrea Petrovic; Suncana Sikiric; Hrvoje Vranes; Andreja Prtoric; Helena Zizek; Tajana Durasin; Ivan Dobric; Mario Staresinic; Sanja Strbe; Mario Knezevic; Marija Sola; Antonio Kokot; Marko Sever; Eva Lovric; Anita Skrtic; Alenka Boban Blagaic; Predrag Sikiric Journal: Front Pharmacol Date: 2021-06-29 Impact factor: 5.810