BACKGROUND: Chronic inflammation and oxidative stress are common features in hemodialysis patients. However, research results on the long-term influence of hemodiafiltration (HDF) on oxidative stress and inflammation are limited. METHODS: We studied changes of inflammatory and oxidative stress biomarkers in 9 stable patients on post-dilution HDF for 9 months. Total antioxidant capacity (TAC), reactive oxygen metabolites (d-ROMs) and superoxide dismutase (SOD) measured by spectrophotometry were used as oxidative stress biomarkers. High-sensitivity CRP (hs-CRP) and IL-6 measured by ELISA were used as inflammation biomarkers. RESULTS: d-ROMs showed significant reduction at 3 months (323.0 +/- 81.9 vs. 275.0 +/- 62.0 U, p < 0.008) and remained low (267.9 +/- 45.8 U) to the end of the study. At the end of the study there was a significant increase in TAC levels (1.3 +/- 0.1 vs. 1.4 +/- 0.1 mmol/l, p < 0.03), while SOD rise did not reach statistical significance (13.1 +/- 9.8 vs. 18.7 +/- 9.4 U/ml, p = 0.06). hs-CRP values at the end of the study decreased significantly (16.3 +/- 11.4 vs. 6.0 +/- 5.1 microg/ml, p < 0.02), while IL-6 levels showed a non-significant reduction (16.9 +/- 9.3 vs. 12.0 +/- 7.3 pg/ml, p = 0.2). CONCLUSION: Our results suggest that post-dilution HDF for 9 months suppresses oxidative stress by rapid lowering of d-ROMs and improves antioxidant capacity by increasing TAC, while it reduces inflammation by decreasing hs-CRP. Copyright 2008 S. Karger AG, Basel.
BACKGROUND: Chronic inflammation and oxidative stress are common features in hemodialysis patients. However, research results on the long-term influence of hemodiafiltration (HDF) on oxidative stress and inflammation are limited. METHODS: We studied changes of inflammatory and oxidative stress biomarkers in 9 stable patients on post-dilution HDF for 9 months. Total antioxidant capacity (TAC), reactive oxygen metabolites (d-ROMs) and superoxide dismutase (SOD) measured by spectrophotometry were used as oxidative stress biomarkers. High-sensitivity CRP (hs-CRP) and IL-6 measured by ELISA were used as inflammation biomarkers. RESULTS: d-ROMs showed significant reduction at 3 months (323.0 +/- 81.9 vs. 275.0 +/- 62.0 U, p < 0.008) and remained low (267.9 +/- 45.8 U) to the end of the study. At the end of the study there was a significant increase in TAC levels (1.3 +/- 0.1 vs. 1.4 +/- 0.1 mmol/l, p < 0.03), while SOD rise did not reach statistical significance (13.1 +/- 9.8 vs. 18.7 +/- 9.4 U/ml, p = 0.06). hs-CRP values at the end of the study decreased significantly (16.3 +/- 11.4 vs. 6.0 +/- 5.1 microg/ml, p < 0.02), while IL-6 levels showed a non-significant reduction (16.9 +/- 9.3 vs. 12.0 +/- 7.3 pg/ml, p = 0.2). CONCLUSION: Our results suggest that post-dilution HDF for 9 months suppresses oxidative stress by rapid lowering of d-ROMs and improves antioxidant capacity by increasing TAC, while it reduces inflammation by decreasing hs-CRP. Copyright 2008 S. Karger AG, Basel.
Authors: Ayşe Ağbaş; Nur Canpolat; Salim Çalışkan; Alev Yılmaz; Hakan Ekmekçi; Mark Mayes; Helen Aitkenhead; Franz Schaefer; Lale Sever; Rukshana Shroff Journal: PLoS One Date: 2018-06-18 Impact factor: 3.240
Authors: Ria Arnold; Bruce A Pussell; Timothy J Pianta; Virginija Grinius; Cindy S-Y Lin; Matthew C Kiernan; James Howells; Meg J Jardine; Arun V Krishnan Journal: PLoS One Date: 2013-03-11 Impact factor: 3.240
Authors: Simonetta Palleschi; Paolo M Ghezzi; Giuseppe Palladino; Barbara Rossi; Marino Ganadu; Domenica Casu; Maria Cossu; Giovanni Mattana; Antonio Maria Pinna; Bruno Contu; Tonina Ghisu; Alessandro Monni; Luana Gazzanelli; Maria Cristina Mereu; Franco Logias; Mario Passaghe; Alessandro Amore; Piergiorgio Bolasco Journal: BMC Nephrol Date: 2016-08-26 Impact factor: 2.388