Lan Chen1, Xiaoping Zhang, Lindstrom Kristina. 1. College of Resources and Environmental Science, Sichuan Agricultural University, Ya'an 625000, China. chenlanmail@yahoo.com.cn
Abstract
OBJECTIVE: The biodiversity of endophytic Bacillus isolated from Medicinal plants was studied. METHODS: Endophytic bacillus isolated from medicinal plants and reference strains were characterized by numerical taxonomy, 16S rDNA PCR-RFLP, BOX-PCR fingerprints and 16S rDNA sequence to describe the phenotypic and genetic diversity and phylogeny. RESULTS: The phenotypic characterization of these strains showed that most of these strains had high stress resistance. There were 13 clusters formed at 84% similarity by numerical taxonomy. The genetic diversity was highly revealed by 16S rDNA PCR-RFLP. The diversity of genomic level was described by BOX-PCR fingerprints. The results were correlated well with numerical analysis. Homology analysis was made by searching in Genbank with NCBI, and the phylogenetic tree was reconstructed. Sequence analysis of 16S rRNA gene showed that SCAU11 was highly related to B. sphaericus, SCAU78 and SCAU25 were identified as B. subtilis subspecies, SCAU39 was highly related to B. megaterium. CONCLUSION: Overall, the study results demonstrated a high phenotypic and phylogenetic diversity of endophytic Bacillus isolated from Medicinal plants.
OBJECTIVE: The biodiversity of endophytic Bacillus isolated from Medicinal plants was studied. METHODS: Endophytic bacillus isolated from medicinal plants and reference strains were characterized by numerical taxonomy, 16S rDNA PCR-RFLP, BOX-PCR fingerprints and 16S rDNA sequence to describe the phenotypic and genetic diversity and phylogeny. RESULTS: The phenotypic characterization of these strains showed that most of these strains had high stress resistance. There were 13 clusters formed at 84% similarity by numerical taxonomy. The genetic diversity was highly revealed by 16S rDNA PCR-RFLP. The diversity of genomic level was described by BOX-PCR fingerprints. The results were correlated well with numerical analysis. Homology analysis was made by searching in Genbank with NCBI, and the phylogenetic tree was reconstructed. Sequence analysis of 16S rRNA gene showed that SCAU11 was highly related to B. sphaericus, SCAU78 and SCAU25 were identified as B. subtilis subspecies, SCAU39 was highly related to B. megaterium. CONCLUSION: Overall, the study results demonstrated a high phenotypic and phylogenetic diversity of endophytic Bacillus isolated from Medicinal plants.