OBJECTIVE: To establish a method for the reliable isolation and culture of infantile hemangioma endothelial cells (HemECs) in vitro. METHODS: Proliferative hemangioma specimens were digested by collagenase to form a single cell suspension. The HemECs were isolated using anti-CD31 coated dynabeads. The CD31+ cells were grown in fibronectin coated dishes. HemECs were identified by morphological characteristics and immunocytochemistry. The cells were also examined for their ability to intake LDL. RESULTS: The method enabled the rapid isolation of HemECs that demonstrated typical endothelial cobblestone morphology in culture. The cells were positively stained for CD31, vWF. They also were labeled with DiI-Ac-LDL. CONCLUSIONS: This technique can effectively isolate endothelial cells from the proliferative hemangiomas. These cells could be further used to research the mechanism of proliferation and degeneration of infantile hemangioma.
OBJECTIVE: To establish a method for the reliable isolation and culture of infantile hemangioma endothelial cells (HemECs) in vitro. METHODS: Proliferative hemangioma specimens were digested by collagenase to form a single cell suspension. The HemECs were isolated using anti-CD31 coated dynabeads. The CD31+ cells were grown in fibronectin coated dishes. HemECs were identified by morphological characteristics and immunocytochemistry. The cells were also examined for their ability to intake LDL. RESULTS: The method enabled the rapid isolation of HemECs that demonstrated typical endothelial cobblestone morphology in culture. The cells were positively stained for CD31, vWF. They also were labeled with DiI-Ac-LDL. CONCLUSIONS: This technique can effectively isolate endothelial cells from the proliferative hemangiomas. These cells could be further used to research the mechanism of proliferation and degeneration of infantile hemangioma.
Authors: Branislava Janic; Kourosh Jafari-Khouzani; Abbas Babajani-Feremi; A S M Iskander; Nadimpalli Ravi S Varma; Meser M Ali; Robert A Knight; Ali S Arbab Journal: PLoS One Date: 2012-05-25 Impact factor: 3.240
Authors: Branislava Janic; Austin M Guo; A S M Iskander; Nadimpalli Ravi S Varma; Alfonso G Scicli; Ali S Arbab Journal: PLoS One Date: 2010-02-11 Impact factor: 3.240