OBJECTIVE: To design a macroporous resin chromatography system with on-line detector which can be used to purify total flavonoid glycosides from Hypericum perforatum. METHOD: Compared with the present detection methods, the ultraviolet detector which can be applied to flow liquid, and a self-made shunt were applied to design the on-line detection system. This system was successfully applied to purify the total flavonoid glycosides from H. perforatum. RESULT: This on-line detection system was simple and feasible, and the results were accurate and reliable. The purity of total flavonoid glycosides from H. perforatum. separated by this system was higher than 90%. CONCLUSION: This technique provides a simple and reliable on-line detection method for industrial chromatography purification of TCM extracts, which is the basis of automatic manufactory of TCM purification.
OBJECTIVE: To design a macroporous resin chromatography system with on-line detector which can be used to purify total flavonoid glycosides from Hypericum perforatum. METHOD: Compared with the present detection methods, the ultraviolet detector which can be applied to flow liquid, and a self-made shunt were applied to design the on-line detection system. This system was successfully applied to purify the total flavonoid glycosides from H. perforatum. RESULT: This on-line detection system was simple and feasible, and the results were accurate and reliable. The purity of total flavonoid glycosides from H. perforatum. separated by this system was higher than 90%. CONCLUSION: This technique provides a simple and reliable on-line detection method for industrial chromatography purification of TCM extracts, which is the basis of automatic manufactory of TCM purification.
Authors: Xueli Cao; Qiaoe Wang; Yan Li; Ge Bai; Hong Ren; Chunming Xu; Yoichiro Ito Journal: J Chromatogr B Analyt Technol Biomed Life Sci Date: 2011-01-21 Impact factor: 3.205