BACKGROUND: The clinical relevance of immunoglobulin E (IgE) to plant glycans is a longstanding debate. We sought to evaluate their clinical reactivity using the human glycoprotein lactoferrin expressed in rice. METHODS: Allergic patients with IgE antibodies against plant glycans were analyzed for the presence of IgE against rice-produced lactoferrin. The potency of IgE to induce mediator release was assessed by basophil histamine release and skin prick tests (SPTs). Clinical relevance was evaluated by double-blind placebo-controlled oral challenge (DBPCOC). RESULTS: Twenty-four of 29 sera (82.7%) with IgE antibodies against plant glycans demonstrated IgE binding to transgenic lactoferrin. In three of five cases transgenic lactoferrin induced histamine release. Compared to a control major grass pollen allergen lactoferrin concentrations needed for biological activity of IgE were 5-6 orders of magnitude higher. Skin prick test and DBPCOC were negative in five patients with potential clinical reactivity that volunteered to undergo these in vivo challenges. CONCLUSIONS: Poor or no biological activity and lack of clinical relevance of IgE-binding plant glycans (five out of five) was demonstrated using human lactoferrin expressed in rice as a model.
BACKGROUND: The clinical relevance of immunoglobulin E (IgE) to plant glycans is a longstanding debate. We sought to evaluate their clinical reactivity using the human glycoprotein lactoferrin expressed in rice. METHODS: Allergic patients with IgE antibodies against plant glycans were analyzed for the presence of IgE against rice-produced lactoferrin. The potency of IgE to induce mediator release was assessed by basophil histamine release and skin prick tests (SPTs). Clinical relevance was evaluated by double-blind placebo-controlled oral challenge (DBPCOC). RESULTS: Twenty-four of 29 sera (82.7%) with IgE antibodies against plant glycans demonstrated IgE binding to transgenic lactoferrin. In three of five cases transgenic lactoferrin induced histamine release. Compared to a control major grass pollen allergen lactoferrin concentrations needed for biological activity of IgE were 5-6 orders of magnitude higher. Skin prick test and DBPCOC were negative in five patients with potential clinical reactivity that volunteered to undergo these in vivo challenges. CONCLUSIONS: Poor or no biological activity and lack of clinical relevance of IgE-binding plant glycans (five out of five) was demonstrated using human lactoferrin expressed in rice as a model.
Authors: Anna Pomés; Janet M Davies; Gabriele Gadermaier; Christiane Hilger; Thomas Holzhauser; Jonas Lidholm; Andreas L Lopata; Geoffrey A Mueller; Andreas Nandy; Christian Radauer; Sanny K Chan; Uta Jappe; Jörg Kleine-Tebbe; Wayne R Thomas; Martin D Chapman; Marianne van Hage; Ronald van Ree; Stefan Vieths; Monika Raulf; Richard E Goodman Journal: Mol Immunol Date: 2018-04-04 Impact factor: 4.407
Authors: Rainer Fischer; Tanja Holland; Markus Sack; Stefan Schillberg; Eva Stoger; Richard M Twyman; Johannes F Buyel Journal: Adv Biochem Eng Biotechnol Date: 2021 Impact factor: 2.635
Authors: Audrey E Koid; Martin D Chapman; Robert G Hamilton; Ronald van Ree; Serge A Versteeg; Stephen C Dreskin; Stef J Koppelman; Sabina Wünschmann Journal: J Agric Food Chem Date: 2013-12-26 Impact factor: 5.279