BACKGROUND: We characterised 37 amoebae cultured from corneal scrapings, contact lenses or lens case solutions of patients with suspected Acanthamoeba keratitis. METHODS: The isolates were identified by their morphology and by PCR targeting the Acanthamoeba nuclear small-subunit rRNA gene. Acanthamoeba isolates were genotyped by DNA sequence analysis. RESULTS: The 37 isolates comprised 35 Acanthamoeba, one Hartmannella and one Vahlkampfia. Ten Acanthamoeba isolates were shown to be responsible for keratitis. CONCLUSION: Genotype T4 was the only Acanthamoeba genotype responsible for keratitis in this study, and represented 79% of non-pathogenic isolates.
BACKGROUND: We characterised 37 amoebae cultured from corneal scrapings, contact lenses or lens case solutions of patients with suspected Acanthamoeba keratitis. METHODS: The isolates were identified by their morphology and by PCR targeting the Acanthamoeba nuclear small-subunit rRNA gene. Acanthamoeba isolates were genotyped by DNA sequence analysis. RESULTS: The 37 isolates comprised 35 Acanthamoeba, one Hartmannella and one Vahlkampfia. Ten Acanthamoeba isolates were shown to be responsible for keratitis. CONCLUSION: Genotype T4 was the only Acanthamoeba genotype responsible for keratitis in this study, and represented 79% of non-pathogenic isolates.
Authors: A Magnet; A L Galván; S Fenoy; F Izquierdo; C Rueda; C Fernandez Vadillo; J Pérez-Irezábal; K Bandyopadhyay; G S Visvesvara; A J da Silva; C del Aguila Journal: Parasitol Res Date: 2012-03-07 Impact factor: 2.289
Authors: D Di Cave; R Monno; P Bottalico; S Guerriero; S D'Amelio; C D'Orazi; F Berrilli Journal: Eur J Clin Microbiol Infect Dis Date: 2008-12-18 Impact factor: 3.267
Authors: D R Ledee; A Iovieno; D Miller; N Mandal; M Diaz; J Fell; M E Fini; E C Alfonso Journal: J Clin Microbiol Date: 2009-03-25 Impact factor: 5.948
Authors: Agnieszka Wojtkowiak-Giera; Monika Derda; Agnieszka Kolasa-Wołosiuk; Edward Hadaś; Danuta Kosik-Bogacka; Piotr Solarczyk; Paweł P Jagodziński; Elżbieta Wandurska-Nowak Journal: Parasitol Res Date: 2016-08-11 Impact factor: 2.289